d(GC) - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 6 (2) 190-195 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)
. Table 2. Effect of xylan concentration on xylanase activity at different time intervals
Time 0% xylan 0.5% xylan 1% xylan 2% xylan 3% xylan 4% xylan 5% xylan
(hours) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml)
12 0.18± 0.01 0.89 ± 0.08 1.34 ±0.13 1.60 ± 0.15 2.05 ± 0.20 3.74 ± 0.35 2.40 ± 0.20
24 0.20 ±0.01 1.16 ± 0.10 2.49 ± 0.20 3.74 ± 0.30 4.45 ± 0.40 4.72 ± 0.40 4.36 ± 0.40
36 0.27± 0.01 1.25 ± 0.12 2.58 ± 0.25 4.18 ± 0.40 5.52 ± 0.50 6.41 ± 0.55 5.25 ± 0.50
48 0.30± 0.01 1.34 ± 0.13 2.76 ± 0.25 4.36 ± 0.40 6.41 ± 0.60 7.21 ± 0.70 6.85 ± 0.60
60 0.10± 0.01 1.42 ± 0.14 2.49 ± 0.24 4.27 ± 0.40 7.03 ± 0.70 8.01 ± 0.75 8.46 ± 0.75
72 0.30± 0.01 1.07 ± 0.10 2.23 ± 0.20 4.18 ±0.40 5.52 ± 0.50 8.54 ± 0.75 8.99 ± 0.80
84 0.10± 0.01 0.89 ± 0.08 0.98 ± 0.08 3.74 ± 0.35 4.81 ± 0.45 7.12 ±0.70 9.08 ± 0.85
observed that there was initially up to 48 hours
increase in xylanase production and thereafter
decrease in xylanase production was observed
(Table 2). When 1% more xylan was added after
48 hours of growth, there was increase in
xylanase production up to 96 hours. The data
indicated that due to extra-cellular xylanase, xylan
present in the medium exhausted and upon
further addition of xylan increased xylanase
production.
The effect of various different
concentrations of xylan viz. 0.5, 1, 2 , 3, 4 and 5
% xylan was also checked on xylan production.
The data are given in Table 2. These data showed
that there was more production of xylanase with
increase in xylan concentration up to 4% in the
growth medium. At lower concentration of xylan,
increase in xylanase production was observed
up to 48 hours only whereas at higher
concentration of xylan, increase in xylanase
production was observed for a longer time (Table
2). The present results showed that although
xylanase isolated from Paenibacillus
macquariensis is not thermophilic but it can be
exploited as good source of xylanase production
for industrial applications.
Acknowledgements
The authors acknowledge the
Department of Biotechnology, Ministry of Science
and Technology, Government of India, New Delhi,
194
India for its facilities under M.Sc. Biotechnology
program and the Bioinformatics Sub Centre.
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