d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 6 (2) 190-195 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
. Table 2. Effect <strong>of</strong> xylan concentration on xylanase activity at different time intervals<br />
Time 0% xylan 0.5% xylan 1% xylan 2% xylan 3% xylan 4% xylan 5% xylan<br />
(hours) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml) (U/ml)<br />
12 0.18± 0.01 0.89 ± 0.08 1.34 ±0.13 1.60 ± 0.15 2.05 ± 0.20 3.74 ± 0.35 2.40 ± 0.20<br />
24 0.20 ±0.01 1.16 ± 0.10 2.49 ± 0.20 3.74 ± 0.30 4.45 ± 0.40 4.72 ± 0.40 4.36 ± 0.40<br />
36 0.27± 0.01 1.25 ± 0.12 2.58 ± 0.25 4.18 ± 0.40 5.52 ± 0.50 6.41 ± 0.55 5.25 ± 0.50<br />
48 0.30± 0.01 1.34 ± 0.13 2.76 ± 0.25 4.36 ± 0.40 6.41 ± 0.60 7.21 ± 0.70 6.85 ± 0.60<br />
60 0.10± 0.01 1.42 ± 0.14 2.49 ± 0.24 4.27 ± 0.40 7.03 ± 0.70 8.01 ± 0.75 8.46 ± 0.75<br />
72 0.30± 0.01 1.07 ± 0.10 2.23 ± 0.20 4.18 ±0.40 5.52 ± 0.50 8.54 ± 0.75 8.99 ± 0.80<br />
84 0.10± 0.01 0.89 ± 0.08 0.98 ± 0.08 3.74 ± 0.35 4.81 ± 0.45 7.12 ±0.70 9.08 ± 0.85<br />
observed that there was initially up to 48 hours<br />
increase in xylanase production <strong>and</strong> thereafter<br />
decrease in xylanase production was observed<br />
(Table 2). When 1% more xylan was added after<br />
48 hours <strong>of</strong> growth, there was increase in<br />
xylanase production up to 96 hours. The data<br />
indicated that due to extra-cellular xylanase, xylan<br />
present in the medium exhausted <strong>and</strong> upon<br />
further addition <strong>of</strong> xylan increased xylanase<br />
production.<br />
The effect <strong>of</strong> various different<br />
concentrations <strong>of</strong> xylan viz. 0.5, 1, 2 , 3, 4 <strong>and</strong> 5<br />
% xylan was also checked on xylan production.<br />
The data are given in Table 2. These data showed<br />
that there was more production <strong>of</strong> xylanase with<br />
increase in xylan concentration up to 4% in the<br />
growth medium. At lower concentration <strong>of</strong> xylan,<br />
increase in xylanase production was observed<br />
up to 48 hours only whereas at higher<br />
concentration <strong>of</strong> xylan, increase in xylanase<br />
production was observed for a longer time (Table<br />
2). The present results showed that although<br />
xylanase isolated from Paenibacillus<br />
macquariensis is not thermophilic but it can be<br />
exploited as good source <strong>of</strong> xylanase production<br />
for industrial applications.<br />
Acknowledgements<br />
The authors acknowledge the<br />
Department <strong>of</strong> <strong>Biotechnology</strong>, Ministry <strong>of</strong> Science<br />
<strong>and</strong> Technology, Government <strong>of</strong> India, New Delhi,<br />
194<br />
India for its facilities under M.Sc. <strong>Biotechnology</strong><br />
program <strong>and</strong> the Bioinformatics Sub Centre.<br />
References<br />
1. Kuhad, R.C., Singh, A. <strong>and</strong> Eriksson, K.E.<br />
(1997) Microorganisms <strong>and</strong> enzymes<br />
involved in the degradation <strong>of</strong> plant fibre cell<br />
walls. Adv. Biochem Eng. Biotechnol 57, 45-<br />
125<br />
2. Collins, T., Gerday, C. <strong>and</strong> Feller, G. (2005)<br />
Xylanases, xylanase families <strong>and</strong><br />
extremophilic xylanases. FEMS Microbiol.<br />
Rev. 29, 3-23<br />
3. Sunna, A., <strong>and</strong> Antranikian, G. (1997)<br />
Xylanolytic enzymes from fungi <strong>and</strong><br />
bacteria. Crit. Rev. Biotechnol. 17, 39-67.<br />
4. Kh<strong>and</strong>eparker, R., <strong>and</strong> Numan, M.T. (2008)<br />
Bifunctional xylanases <strong>and</strong> their potential<br />
use in biotechnology. J Ind. Microbiol,<br />
Biotechnol. 35, 635-644.<br />
5. Prade, R.A. (1996) Xylanases: from biology<br />
to biotechnology. Biotechnol. Genet. Eng.<br />
Rev. 13, 101-131.<br />
6. Wong, K.K.Y., Tan, L.U.L. <strong>and</strong> Saddler, J.N.<br />
(1998) Multiplicity <strong>of</strong> β-1,4-xylanase in<br />
microorganisms, functions <strong>and</strong><br />
applications. Microbiol. Rev. 52, 305-317.<br />
7. Kulkarni, N., Shendye, A. <strong>and</strong> Rao, M.<br />
(1999) Molecular <strong>and</strong> biotechnological<br />
Optimization <strong>of</strong> Xylanase Secretion from Paenibacillus macquariensis