d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 6 (2) 173-182 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
system is easy in operation, locus specific <strong>and</strong><br />
works uniformly with all the isolates. The speed,<br />
simplicity <strong>and</strong> reliability <strong>of</strong> PCR based<br />
approaches make microsatellite analysis on<br />
agarose gels an attractive tool for MAS in rice<br />
breeding programs aiming at developing durable<br />
rice blast resistant cultivars (27), SSRs also the<br />
marker choice for genomic analysis for many<br />
crop species (28).<br />
Moreover, we observed the higher PIC<br />
scores for MGM markers in compared to the Pot2<br />
primers, which indicate that these markers were<br />
better suited for underst<strong>and</strong>ing the genetic<br />
diversity in blast fungus populations.<br />
MGM based genetic relationship: Cluster<br />
analysis <strong>of</strong> the blast isolates revealed the average<br />
pair wise similarities in the range <strong>of</strong> 0.15 to 0.9<br />
suggesting large variations among the isolates<br />
<strong>of</strong> the present study. The observed diversity level<br />
was much higher than that <strong>of</strong> an earlier study<br />
(24). This could be because the present study<br />
176<br />
involved isolates collected from different hotspot<br />
regions <strong>of</strong> India, while the earlier study used only<br />
the isolates collected from Northwestern<br />
Himalayan region <strong>of</strong> India. All the isolates were<br />
grouped into two major groups (Fig. 2). Cluster–<br />
I consisted <strong>of</strong> 29 isolates, which were further<br />
divided into two sub clusters (IA <strong>and</strong> IB). The sub<br />
cluster IA consisting <strong>of</strong> four isolates in that, two<br />
isolates (SP 5 <strong>and</strong> SP 6) were collected from<br />
DRR <strong>and</strong> another two isolates were collected<br />
from Nawagam (SP 27 <strong>and</strong> SP 28) <strong>and</strong> all the<br />
isolates <strong>of</strong> this sub cluster shares 32 % similarity.<br />
Whereas, sub cluster IB was comprised <strong>of</strong> 16<br />
isolates, among which 7 isolates were collected<br />
from DRR <strong>and</strong> rest were collected from different<br />
blast endemic areas <strong>of</strong> India, all the isolates <strong>of</strong><br />
this sub cluster have the combined similarity <strong>of</strong><br />
35 %. Whereas, the second major cluster<br />
consists <strong>of</strong> 5 isolates, among which two isolates<br />
were collected from Palampur <strong>and</strong> 3 isolates<br />
were collected from DRR, all the isolates in this<br />
cluster showed only 15 % similarity.<br />
Fig. 2. Dendogram constructed from MGM <strong>and</strong> Pot2 primers based fingerprint data from 34 rice infecting <strong>of</strong><br />
Magnaporthe grisea isolates from India. (ALM- Almora, ASM- Assam, DRR- Directorate <strong>of</strong> Rice Research,<br />
JGY- Jagityala, KJT- Karjat, MDY- M<strong>and</strong>ya, MTU- Mareturu, NLR- Nellore, NWG- Nawagam, PLP - Palampur,<br />
RNC - Ranchi).<br />
Madhan Mohan et al
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