d(GC) - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 6 (2) 145-165 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)
case of acetone and formaldehyde only 49 and
12% of residual activity was retained. The effect
of different solvents at two levels (20 and 60% v/
v) was tested (9) and it was found that at 60% of
concentration, ethanol and acetone completely
inhibited the tannase activity, whereas
tetrahydrofuran and formaldehyde exhibited
inhibitory effect only at 20% (v/v). Propanol acts
as an activator in the range of 3.6 - 7.3% (v/v),
but higher concentration inhibits the propyl gallate
synthesis by denaturation of tannase (100).
Stability in organic solvents suggests suitability
of tannase for synthetic reactions, and opens new
path to synthesize novel compounds of
pharmaceutical interest.
Conclusions
This review focuses on the recent
advances on the scientific and technological
aspects of tannase fermentation techniques
(SmF, SSF and LSF) and downstream
processing. Recent advances in submerged and
solid state fermentation of tannase bioprocessing
have been explained thoroughly. Additionally this
review includes a list of agro-industrial residues
used for high yield cost effective fermentation of
tannase. A detailed depiction of physicochemical
characterization like pH and temperature
stability, stability in non-aqueous solvents, effects
of metals and ions, surfactants and denaturants
including special features represents a
comparative and unique collection of information
for future researchers and industrial need.
Moreover this review gives clear idea of future
perspectives on bioprocessing strategies to
reduce the production cost of tannase by
using agro-industrial residues and increased
feasibility of scale-up studies towards
commercialization.
Acknowledgment
Council of Scientific and Industrial
Research (CSIR), India is duly acknowledged for
Junior and Senior Research Fellowship awarded
to Dinesh Prasad.
Dinesh Prasad et al
158
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