d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
The three-dimensional graphs were<br />
generated for the possible combination <strong>of</strong> the<br />
three factors. Graphs for interactions are<br />
revealed here to highlight the roles played by<br />
these factors (Fig. 4). From the central point <strong>of</strong><br />
the contour plot the optimal process parameters<br />
were identified.<br />
Effect <strong>of</strong> amino acids as a stimulator for GSH<br />
production: The effect <strong>of</strong> cysteine on GSH<br />
production was shown in Fig. 5. GSH production<br />
increased to 163.12 mg/L with the addition <strong>of</strong> 3<br />
Fig. 5. Effect <strong>of</strong> cysteine on GSH production<br />
Fig. 6. Effect <strong>of</strong> amino acids on GSH production<br />
Enhanced Production <strong>of</strong> Glutathione<br />
251<br />
mM cysteine simultaneously biomass<br />
concentration was found to be decreased with<br />
cysteine addition. The inhibition could be through<br />
metal chelation <strong>of</strong> specific enzymes concerned<br />
with carbohydrate metabolism. Alfafara et al. (23)<br />
studied the effect <strong>of</strong> amino acids on GSH<br />
production <strong>and</strong> reported cysteine as the key<br />
amino acid for GSH production. Fig. 6 shows the<br />
effect <strong>of</strong> glycine, glutamic acid, methionine,<br />
serine, tyrosine <strong>and</strong> lysine on GSH production.<br />
Glycine, glutamic acid, <strong>and</strong> methionine increased
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