d(GC) - Association of Biotechnology and Pharmacy

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Current Trends in Biotechnology and Pharmacy Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online) Table 3. Predicted and experimental yields of GSH by validation Sr. No Optimum concentrations (%) GSH (mg/L) Glucose Yeast extract MgSO 4 Predicted Experimentally verified a 1 5.67 7.13 0.66 149.65 148.45 ± 1.031 2 5.68 7.14 0.66 149.65 148.38 ± 1.113 3 5.73 7.19 0.66 149.65 147.92 ± 1.091 a Results are mean ± SD of at least three determinations (A) Effect of yeast extract and glucose when other variables are held at zero level (B) Effect of magnesium sulphate and glucose when other variables are held at zero level Fig. 4. Contour plot and surface plot for GSH Parbatsingh Rajpurohit et al 250 (C) Effect of magnesium sulphate and yeast extract when other variables are held at zero level
Current Trends in Biotechnology and Pharmacy Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online) The three-dimensional graphs were generated for the possible combination of the three factors. Graphs for interactions are revealed here to highlight the roles played by these factors (Fig. 4). From the central point of the contour plot the optimal process parameters were identified. Effect of amino acids as a stimulator for GSH production: The effect of cysteine on GSH production was shown in Fig. 5. GSH production increased to 163.12 mg/L with the addition of 3 Fig. 5. Effect of cysteine on GSH production Fig. 6. Effect of amino acids on GSH production Enhanced Production of Glutathione 251 mM cysteine simultaneously biomass concentration was found to be decreased with cysteine addition. The inhibition could be through metal chelation of specific enzymes concerned with carbohydrate metabolism. Alfafara et al. (23) studied the effect of amino acids on GSH production and reported cysteine as the key amino acid for GSH production. Fig. 6 shows the effect of glycine, glutamic acid, methionine, serine, tyrosine and lysine on GSH production. Glycine, glutamic acid, and methionine increased
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