d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
d(GC) - Association of Biotechnology and Pharmacy
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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online)<br />
source supported the maximum GSH production<br />
61.87 mg/L (biomass, 5.39 g/L). Santos et al. (18)<br />
reported glucose as a sole carbon source for<br />
maximum production <strong>of</strong> GSH (154.5 mg/L) using<br />
S. cerevisiae ATCC 7754. Liu et al. (14) <strong>and</strong> Cha<br />
et al. (21) screened various carbon sources for<br />
production <strong>of</strong> GSH using S. cerevisiae ATCC<br />
7754 <strong>and</strong> S. cerevisiae FF-8 respectively, found<br />
glucose as the best carbon source for maximum<br />
GSH production as 115.28 mg/L <strong>and</strong> 204 mg/L<br />
respectively.<br />
Effects <strong>of</strong> nitrogen sources : The effects <strong>of</strong><br />
nitrogen sources on GSH production <strong>and</strong> cell<br />
growth by S. cerevisiae NCIM 3454 were shown<br />
in Fig. 2. Yeast extract supported maximum GSH<br />
production, 70.96 mg/L (biomass, 6.18 g/L) <strong>and</strong><br />
followed by peptone, 62.32 mg/L (biomass, 5.54<br />
g/L). Hence, yeast extract was chosen as a<br />
nitrogen source for further studies. Sodium nitrate<br />
was found to be the best among inorganic<br />
nitrogen sources screened with GSH production,<br />
44.9 mg/L (biomass, 3.86 g/L). Previously, Cha<br />
et al. (21) studied the effect <strong>of</strong> various organic<br />
<strong>and</strong> inorganic nitrogen sources on GSH<br />
Fig. 3. Growth curve <strong>and</strong> production pr<strong>of</strong>ile <strong>of</strong> GSH<br />
Enhanced Production <strong>of</strong> Glutathione<br />
247<br />
production using S. cerevisiae FF-8. Maximum<br />
GSH production <strong>of</strong> (204 mg/L) was found in<br />
presence <strong>of</strong> 3 % yeast extract as nitrogen source<br />
followed by tryptone (67.4 mg/L). Liu et al. (14)<br />
reported peptone (4.84 %) to be the best nitrogen<br />
source for GSH production (115.28 mg/L) using<br />
S. cerevisiae ATCC 7754. Rollini & Manzoni (22)<br />
studied the effect <strong>of</strong> different fermentation<br />
parameters on GSH volumetric productivity by<br />
S. cerevisiae CBS 1171 <strong>and</strong> reported ammonium<br />
sulphate to be best nitrogen source for GSH<br />
volumetric productivity.<br />
Growth curve <strong>and</strong> production pr<strong>of</strong>ile: The<br />
growth curve <strong>and</strong> production pr<strong>of</strong>ile <strong>of</strong> the GSH<br />
using S. cerevisiae NCIM 3454 were carried out<br />
with respect to time (Fig. 3). The production <strong>of</strong><br />
the GSH was observed from 6 h <strong>of</strong> fermentation<br />
(20.66 mg/L) <strong>and</strong> reached a maximum at 48 h<br />
(128.28 mg/L). Dry cell weight (DCW) was also<br />
found to be maximum (10.78 g/L) at the end <strong>of</strong><br />
48 h. GSH production <strong>and</strong> biomass concentration<br />
did not change at 54 h <strong>and</strong> 60 h indicating that<br />
the organism might have reached the death<br />
phase.