d(GC) - Association of Biotechnology and Pharmacy

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Current Trends in Biotechnology and Pharmacy Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online) g/L) was observed. It was found that GSH production along with biomass concentration was lesser than all pH values other than 6.0; hence pH was found to be a significant factor for GSH production. The results are found in accordance with Santos et al. (18). They reported maximum production of GSH, 60.5 mg/L (after 24 h) at an initial pH of 6.0 for using S. cerevisiae ATCC 7754. Fig. 1. Effect of carbon sources 246 Effects of carbon sources: As during fermentation process, carbon source plays dual characteristic role, by acting as major constituent for building of cellular material and as an energy source (19, 20). Fig. 1 shows the effect of different carbon sources on GSH production. None of the carbon sources increased the production further. Glucose as a sole carbon Fig. 2. Effect of nitrogen sources Parbatsingh Rajpurohit et al
Current Trends in Biotechnology and Pharmacy Vol. 6 (2) 241-254 April 2012, ISSN 0973-8916 (Print), 2230-7303 (Online) source supported the maximum GSH production 61.87 mg/L (biomass, 5.39 g/L). Santos et al. (18) reported glucose as a sole carbon source for maximum production of GSH (154.5 mg/L) using S. cerevisiae ATCC 7754. Liu et al. (14) and Cha et al. (21) screened various carbon sources for production of GSH using S. cerevisiae ATCC 7754 and S. cerevisiae FF-8 respectively, found glucose as the best carbon source for maximum GSH production as 115.28 mg/L and 204 mg/L respectively. Effects of nitrogen sources : The effects of nitrogen sources on GSH production and cell growth by S. cerevisiae NCIM 3454 were shown in Fig. 2. Yeast extract supported maximum GSH production, 70.96 mg/L (biomass, 6.18 g/L) and followed by peptone, 62.32 mg/L (biomass, 5.54 g/L). Hence, yeast extract was chosen as a nitrogen source for further studies. Sodium nitrate was found to be the best among inorganic nitrogen sources screened with GSH production, 44.9 mg/L (biomass, 3.86 g/L). Previously, Cha et al. (21) studied the effect of various organic and inorganic nitrogen sources on GSH Fig. 3. Growth curve and production profile of GSH Enhanced Production of Glutathione 247 production using S. cerevisiae FF-8. Maximum GSH production of (204 mg/L) was found in presence of 3 % yeast extract as nitrogen source followed by tryptone (67.4 mg/L). Liu et al. (14) reported peptone (4.84 %) to be the best nitrogen source for GSH production (115.28 mg/L) using S. cerevisiae ATCC 7754. Rollini & Manzoni (22) studied the effect of different fermentation parameters on GSH volumetric productivity by S. cerevisiae CBS 1171 and reported ammonium sulphate to be best nitrogen source for GSH volumetric productivity. Growth curve and production profile: The growth curve and production profile of the GSH using S. cerevisiae NCIM 3454 were carried out with respect to time (Fig. 3). The production of the GSH was observed from 6 h of fermentation (20.66 mg/L) and reached a maximum at 48 h (128.28 mg/L). Dry cell weight (DCW) was also found to be maximum (10.78 g/L) at the end of 48 h. GSH production and biomass concentration did not change at 54 h and 60 h indicating that the organism might have reached the death phase.
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