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76 dod dcfafied dce polishings wde compred.s flrbst ar4 for femanbrjon by Ar@hniots tp.cies- Tfu tv prctei! contors of ric. DotishirSs rft.r f.moralion nay be seen i! Table 5. Tl. analysis of variance ofrhe dala h shown in Apperdix vl. ltre .ornparison revealed non signiticdr diffem@ b€twM ihe biomass proteins oftwo tnes ofrice polishings. Th. result! were encouraging. Cheap d€falted fice polishings reptac€d ccily undeflned .ie polishings. A @mp@bte rue prclcin yietd of biomss with dcfatted and undefauetd ric. polishings (17.20 vs t?.33%) was obtained. Direrenl wo*ers have slso used defaned potishinSs a! subsrrare and obrained biomass prot€in yiefds of Z2.l3oh \|ith Andida ulilit (Kini, l9E9), 24.80% with Trichodema hdziaaM (Khe, 1990) ed tj.33o/o with ,lrrchaiotu, ,pe.i.s (Molr'nood er 41., t990). The biohoss prcrein yi.ld by fem.nriry d€fa(ed ricc polishings by Artuhniotts speciq is in confimity wifi rh. prcvioB work. Ir was lhe.eforc decided to use def.tr€d ricepolishings in furtncr cxpea,nen6. 2. Effect ofAcid Trcatmena ofSubstrate Th€ subslral€ should have maximum f€ffen1a1ion in rte D€diun. Fo. this purpose lhe substnte ar€ trsred in diffmnr ways, viz. physicrt or chemicll. Physical treahenrs degrade rhe subsral. at ce utar level while chenicat rrearn€nG help in deending fte chemical bonds. Exkemes ot pH play majo. role in this Espccr. The d.faned rie polishings wss lr@ted wirh 5% HrSOl in a 6rio of t:l (Vv, subsrale : HrSO4 ) and auroctav€d .l l2loc for 30 minules. The lrealed s w.ff .s untreaLd substratcs were ferm€oted with Arehiiot6 specif \nd.r previously optimired condirions. Th. dara on uuc proteiD conrent ot hrrysrcd bionus, (Table 5)was subj€cted ro stalkrical snalFis (Appendix VII). t.he analysis of varianc€ of thc data ftvcded Don significanr diff.i€nc€ in biomN prorein yi.ld oI the acid lrearcd substrale as compared wirh unrr.ared.
ablc 5. Mo t SE lde p.or€in production s affect€d by u stng Atu h,iotu sp. UMcfan.d ri€ Dolishinss 5t.t5 t7.tl ltiircilo|s!b!|d|. 2 11 56,75 57_0J s5,t96 t6.870 r4 o87ro.,2rd 23.E81 l3.9tl 1.125 25:- r.58 20: I t.08 t2,76 , rE or rcotrpn or por.s.r (HB) uYss d eonbn or (sL (y, 5r 31,19 17.197i0.s1. I 57.31 53.19 I5 tt_t8710:t" 13.95 2.0 l7.21 2.5 3.0 24.70 ,0'o0 ---l.r-r 56.72 53.00 17.05 5599 72 r 7.09 i 56.16 - - t?Jt- --r 54.t7 17.22 t1.21 J8.00 sDp.Bci,p! otr @inr h . .otum *hhtn .mh ondiG;;;w sisntf6a(p
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t-,/rrr',r47c
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To The Controller of Examlnations,
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ACKNOWLEDCEMONTS . ll nnie5 lor All
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PreDrmtio. oflooculum for Bt@iba.te
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effecled by addition of I % nolasse
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LIST OF FIGUERS Flturc Tltl. Prr. I
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Chapter - I INTRODUCTION Tle grcwin
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BrcvibrctetiM llavM. Bretibacteriw
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Hc repon€d non signifi@l difcr.nc
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under a sel of condilions: acid str
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Asperyila Iufligarts.l\e @culture s
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20h ofculturins md 44.3 % rne.4Eh f
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ll tcchnique allowed r 54% incrcase
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l5 nunber of yeast c.ll in 10-14 h
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t1 Yogo er al. ( 1976) krmentql Cat
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l9 dd yeasi exkact in con@nrntions
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2l Mahmood ?/ at (1990) sludicd lhe
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2J t'oda d al. ( | 971) Iirmented s
Page 37 and 38: 'neal 25 Ba.jrach.rya and Mudse( (1
Page 39 and 40: 27 Sandhu 4d woraich ( 1983) sludi
Page 41 and 42: 29 Ilashini (1987) srudied the prod
Page 43 and 44: 3l in.lbalion- ]l'ey obseNed that v
Page 45 and 46: ll substrate in balrl n.dium ar pH
Page 47 and 48: )5 Jin €r al. (1994) succeed€d
Page 49 and 50: J7 nported lllat lhc f€mentation
Page 51 and 52: l9 Burgets (1994) dcveloped the mut
Page 53 and 54: Okmolo (1997) rcported that L-lipro
Page 55 and 56: 4i markedly inll €nced fic cGcuhu
Page 57 and 58: 45 Dods ed Kuns (f969) had5ted th.
Page 59 and 60: Ljpiosky and Litchfield (1974) repo
Page 61 and 62: srain of Cellulonore specie!. They
Page 63 and 64: 5l Enriquez and Rodriquez (1983) co
Page 65 and 66: 5l body weight gain werc rcduced ro
Page 67 and 68: 55 co'nnercial diet. Feed consumpri
Page 69 and 70: Chapter - 3 5l MATERIALS AND METHOD
Page 71 and 72: 3. Preparation of in oc|uhm for Ara
Page 73 and 74: 4.4 Setting CarboD to Nit.ogen Rati
Page 75 and 76: I'hrr L Sl,oni'r I lr'csll culfo.c
Page 77 and 78: 65 Alter 48 houls it€ nrmb€r of
Page 79 and 80: 6,1 Autoclaved Vs UnNutoclaved Firs
Page 81 and 82: 69 X:-Xr gP = Fx YPlx q, = p xYx/s,
Page 83 and 84: 1l rll $. rariom werc.djuied accord
Page 85 and 86: 1J opetrirr8 rheir abdomisal cavili
Page 87: '15 A. TO OPTIMIZE THE GROWTH CONDI
Page 91 and 92: 3. DetermiDation of Optimum L€yel
Page 93 and 94: EI I1*as obs€Ned tat !t l% levcl
Page 95 and 96: 83 periods during fmenlatioo fo! pr
Page 97 and 98: E5 Phac lI1. Showib rotrrv lhrker p
Page 99 and 100: 87 candido utilis and ewn dqty linc
Page 101 and 102: 89 Plat€ V. Showins fermcntalion
Page 103 and 104: L 2. l. 4. 5. 9l Overview for A- ar
Page 105 and 106: t] biomass harvested unde. two cond
Page 107 and 108: 95 haN*ted aner 72 houB of ferm€n
Page 109 and 110: O.,4dvrm in srnerghn wirh the yeasr
Page 111 and 112: 99 averae. true protein valu4 of th
Page 113 and 114: 0l Growth Kinetics For l}te explan.
Page 115 and 116: I ; 60 50 40 30 20 10 0 3 2 1 0 -1
Page 117 and 118: 105 C. NUTRITIONAL EVALUATION OF BI
Page 119 and 120: lnniring diDo acid es wa rcported b
Page 121 and 122: t09 Similar ro fed consumpriotr the
Page 123 and 124: 2.5 Protein Elliciency Ratio (PER)
Page 125 and 126: I tl l'hte Vlll Showi'rg cxperincnr
Page 127 and 128: ||5 SUMMARY Rice polishings is a by
Page 129 and 130: |7 Dds lbdned was 0.5 ed 0.7013 for
Page 131 and 132: lt9 BIBLIOGRAPHY AbdelHanreed,N.A.A
Page 133 and 134: l2l Beneke,E.S. 1955. M€dical Myc
Page 135 and 136: l2J Daghir,N.J. and J-L.SeX. 1962_
Page 137 and 138: 125 Cimnl,R.F.. M.ElSawy, E.M.R.mad
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127 I lubbell.C.H. I 990 Feed Stuff
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129 .ohp.$r&. Appl.Miqobiol.Biot ch
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llt Panaiobv,K.ll., A.Ar.v, L.Damyd
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ShojaoBdati.S-A. .nd M.N.chazlj. 19
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135 V"[OS$ !?3.. Pldir dt br of nrt
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NuEicn crudc Fibr. EIbE;d N.F.E C.l
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oil !5.00 ":oo lz0o 3300 2200 2500
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A!?.dir-vl ADlti. of n l@.fin turtl
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. sbdfqd (Pd.ot) IrlS Ald)rir of v.
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t45 AFadix-X: Ad'tndv'.ic.ofd-dwirr
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147 Alp.ldix-Xll ADdrrir of!{drn .
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App6dix-XIV: Aidyrk ofwidc. oflh. d
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ADFft-XU: A!.Bd. ortrlF turhr.&.r o
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Apt odit-xvle Arttn of whE ofrbdr-o
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I5J Ap'codtx.lo( A.d}n ofyrirE ofdd
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t57 Ar!.odir-)Ofil ofrrttt4. oftb d
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. 3i9i6cd1(P4.0t) z 0.ml 159 0.631
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I6l isnir.d in lhc numc tumac€ a(
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3, Eslidlr|iotr ofcrrbotr l6! 'nc f
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165 4. Estimation ofTrue Protein (T
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t67 (5 mL) m'C for 15 ninurq and ca
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169 added and lhe volurnes werc mad
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