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76<br />
dod dcfafied dce polishings wde compred.s flrbst ar4 for femanbrjon by<br />
Ar@hniots tp.cies- Tfu tv prctei! contors of ric. DotishirSs rft.r f.moralion<br />
nay be seen i! Table 5. Tl. analysis of variance ofrhe dala h shown in Apperdix<br />
vl. ltre .ornparison revealed non signiticdr diffem@ b€twM ihe biomass<br />
proteins oftwo tnes ofrice polishings.<br />
Th. result! were encouraging. Cheap d€falted fice polishings reptac€d<br />
ccily undeflned .ie polishings. A @mp@bte rue prclcin yietd of biomss with<br />
dcfatted and undefauetd ric. polishings (17.20 vs t?.33%) was obtained. Direrenl<br />
wo*ers have slso used defaned potishinSs a! subsrrare and obrained biomass<br />
prot€in yiefds of Z2.l3oh \|ith Andida ulilit (Kini, l9E9), 24.80% with<br />
Trichodema hdziaaM (Khe, 1990) ed tj.33o/o with ,lrrchaiotu, ,pe.i.s<br />
(Molr'nood er 41., t990). The biohoss prcrein yi.ld by fem.nriry d€fa(ed ricc<br />
polishings by Artuhniotts speciq is in confimity wifi rh. prcvioB work. Ir was<br />
lhe.eforc decided to use def.tr€d ricepolishings in furtncr cxpea,nen6.<br />
2. Effect ofAcid Trcatmena ofSubstrate<br />
Th€ subslral€ should have maximum f€ffen1a1ion in rte D€diun. Fo.<br />
this purpose lhe substnte ar€ trsred in diffmnr ways, viz. physicrt or chemicll.<br />
Physical treahenrs degrade rhe subsral. at ce utar level while chenicat rrearn€nG<br />
help in deending fte chemical bonds. Exkemes ot pH play majo. role in this<br />
Espccr. The d.faned rie polishings wss lr@ted wirh 5% HrSOl in a 6rio of t:l<br />
(Vv, subsrale : HrSO4 ) and auroctav€d .l l2loc for 30 minules. The lrealed s<br />
w.ff .s untreaLd substratcs were ferm€oted with Arehiiot6 specif \nd.r<br />
previously optimired condirions. Th. dara on uuc proteiD conrent ot hrrysrcd<br />
bionus, (Table 5)was subj€cted ro stalkrical snalFis (Appendix VII). t.he analysis<br />
of varianc€ of thc data ftvcded Don significanr diff.i€nc€ in biomN prorein yi.ld<br />
oI the acid lrearcd substrale as compared wirh unrr.ared.