t-,/rrr',r47c

70
adjrKed at 7.0 wirh lN NaoH md temperaturc of thc fermenrer ws adjuned at 3?
'C. l lre fermenter was then in@ulal€d with fresh jnocnlu it of B.Iavuh @ 5% ot
the gorv(h medium. Again fernenrer \yas run at sp€ed of 100 lpm atong *ith
contntuous supply of fresh nltqed ah. The pll of lhe mcdiun wN checked and
adjstcd al ?.0. tq 24lou6 offtc addition ofsecond inocullm o. E0 hou6 of
toral lbrmedtation pe.iod the biomds was rccovercd aner 15 minlles stcamirg.-the
biomGs wa then dried in llal tra)s in lbrced air oven at65oC.
Following eoc pbclice th€ f@.rls was run five rimes sd rh€
biomass rccovered wa dried and g.ound. It was aratyzed for crud. protein, crude
fibre, erher extracr, .sh md NFE following AOAC (1990) DiomN was
'nethods.
aho analyzed rof lrue protein (Munro and Fteck, 1966), amino acid profil€ (Moore
and SGi\ 1954), Calciu'n sd Phosphorus (Anony'nous, 1954) dd RNA conrenls
(Muro.nd Fleck. 1966).ltese ealysis ee shown in Appendixlr ort llr.
9. Biological Evaluation
Fo! biological cvahadon rhe biomass wd fcd ro 7-days old broil€r
chicks for a period of 9 days. The triat ws @lducted.t Raja Mrham,nad Alcarr
AniDal Nur.iton Rcs@h Cenre., Univ€Firy of^sricultur.. Fais,labad. Eighrccn
sepdalc pens werc construclcd and birds we.€ placed on {ire net fo. rhe collecrion
olrhen droppings on rhe plaslic sheets plac€d on $e fl@r. h this tial fish meat of
the b.sal €tiotr was replaccd with thc biomass at 0,25,50, ?5 and t00 percenl
lelels oD protein equiv.lenl basis.
9.1 Experimental Rations
Six cxperimenlal ralions viz., A, B. C, D, E and F wcrc prepared.
Ration l: was prolcin free lo derermin€ endogenous |lilrcgo loss6. Ration A had no
bioDass ond sewed as conrrol.In ralions, B. C, D and E the quarlityoffish mealot
conrrol was replaccd yith biomss prorein on prorein equiv.tcnr bajs a.d rhese
conlaincd 2.5, 5.0. ?-5 @d 10.0 percent bioh.ss, r.specrively, nE nnrient t€vels in