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69<br />
X:-Xr<br />
gP = Fx YPlx<br />
q, = p xYx/s, whcre,<br />
& = Final Producl<br />
Xr = InilialProduct<br />
la& = lng naltrnl<br />
P = SPecilic growth rate<br />
Y'i" = Gm3 ol microbial ccll produc.d/g sublrate utilized<br />
Ypis = cItm of pmduct flm€dg of subslrat€ ulilizcd<br />
Yp* = Gram! ofproducr rormed/s of c€ll forn€d<br />
Qp<br />
qs<br />
S) - s' =<br />
X: - Xr =<br />
P: - Pr =<br />
SPecific product quotienl i e I<br />
Specific subsrrare uptake rate<br />
Diflcrctre in sub$rakJh'<br />
Difleren(e;n c.llprcducliotr/h '<br />
Diffcrence in Product/h '<br />
br - br = Difl.r€nce in Time<br />
producug @ll/h<br />
as = volumekic rale ofsubstmle utilization Gnrh)<br />
a, = volum.rric iat€orcellma formation (tt4r)<br />
a"<br />
Vol@.tric mle ofproduc. fomrtion (8/Uh)<br />
8. Large Scale Production ofBiomass<br />
Large sol. femcntalion wd (sied out in Iwe litr. @Pacit) glass<br />
fementer. Th. go$'th medium eas prcparcd in fernent r, it wd then autoclaved al<br />
l2l"C for 45 ninutes. Anq auto.lavinS $e pH of the mediun was adiusted al4 0<br />
with lhc help of lN H1SO. added drcp wise silh the help of peruslaltic pumP Thc<br />
leDperalur€ of $e fef,ncnler wr! adjuslcd al 306C, by circulatinS water in the oucr<br />
jacket of the fmenter lftei ehich the 8ro*1h medim sas indulaled *ith frcsh<br />
inocn'r,m of lreluiota tp..s.plically @ 5%. Tle femente. rls tun at 300 rpm<br />
speed !lo!s with contituou supply of frcsh filtd€d air. Tte pH of the mcdium was<br />
checked md adjusted at 4.0. After 56 hours of fcrm€nlation lhe f€rmenler was onc.<br />
again auloclav.d at 12 I 'C for 45 ninulcs dd before 3tad of autoclave y6( sludSe<br />
was add€d 1o the nedium @ l%. Ancl autoclaving tl|c PH of lh€ mcdium was
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