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6,1 Autoclaved Vs UnNutoclaved First Step Fermentation
61
Ar expquneni w6 conducled ro decide whelhq first and second
organisms should be growD in synerSism or one aner $e olher_ For this pufpose six
flasks haNeued aner fi6l phase of fcmemadon eere divided in lwo groups. Out of
these, ttuee flatls wcre adjusled for their pH ar 7.0 rnd then aur@lavcd whe.eas rhc
.emainin8 were adjusred ar pH 7_0 wirh srsitized tN NaoH d.plicalty wilhour
autocraving. AII the fldks werc inoculated wirh inoculum ot B.FlMn asepti.a|y
and $en incubated ^t tl"C tot 12 hours. Atter 72 hours all rhe flasks were
hanesled md lhcir tiue prot€in contenb w.re d€brmined. Tte opdnum condnion
so achieved ws followed in subsequmt expcrimenls.
6.2 Addition of Different Levels of Molasses at 48rh Hour of
F et mentation vriah B.llavum
for improvjng lhe prcduction of bionss prclei! during s€cond phase
of fennenhdon. ir ws deided to supplenenr lhc 8rcw|n medium wilh notas€s.
For Ois purpo*, sir levck ofmolases were 1sr€d viz, l. 2. 3,4, 5, and 6 D.rccnr
and add.d at 48'r hour of g.owth of B.Jtdwn and hanesr€d aner 72 houu of
rermentatjon. The tevet of mola$es giving maximum prolein was used in the
subsequenl expedmenb.
6,3 Addition.of Different Levels of clucose at Different
Time Periods
For rhis expsjment rwo lerels ofglucce ,2., Z ed 4 p€rcenl veE
rested. Tlse tevets werc resred fo. rhree rime periods vz., 0, 24 ed 48 ho!6 of
incub.[on. The oprimum l.vel of ootarss and rime or ternentdtion giving
maxnnum producrion of bion.ss p.olein was us.d in subsequerl experihenrs.
6.4 Comparison ofGlucose and Gurrh
To d@ide if gurh @n rcplacc gtucose ar s,mitd
w.s conducred in whicn gtucose sid gurh w.rc supplemenrcd