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4.4 Setting CarboD to Nit.ogen Ratio<br />
An€. dcrenninalior ofop num lev.lof molscs !o b. added ar 4E(<br />
hours of ferne.rarion, anorher €xp.lim.nr was conducled for dcrerninin8 the<br />
opliDnn carbon to niroSen iatio to decide how mLlch nilrogcn is to be added along<br />
with the addjtior of motarses. For fis purpose five differenl C ratios viz.,27:1,<br />
20:1, 15:1, l0:l and 5jl werc tcst€d for addiiion to rhc Brofin nedium al48 ho!6.<br />
The opdminm cs6on lo nitroSe! rario obffed in rhrs expdimenl was used in<br />
subsequenr €xperiments fo. ,naximum producrion of b,omass prcrem.<br />
4.5 Setting time for Addition of Molasses<br />
FollowinA $e optimu,h l.vel of molsses ro b. add.d ard .ftd s€ninc<br />
C/N 6rio, uorh.r cxpcrimcnr war @nducred lo deide rhe rime ot incubarion ;<br />
vhich this con€nkarioo of motrscs wd to b. add€d. For this purpose rhrcc fim6<br />
ofincubation were setecred viz.,48,60. and 72 houB. Alt the fl.sks were harlscd<br />
alter 96 houls of f.rn.nhrion. Ttlc olrimum incubal<br />
experim€'r fo, addition of mo'",,* -", r"r"-"r," Jil::,":ffil:* -"<br />
4.6 Addition of Corn Steep Liquor (CSL) at 4g Hours<br />
An qpenmed was conducred ro rest thc tevel of CSL to be added in<br />
the femenblion medium ar 48 hou6 of incubation for moxmum yjetd of biojrsss<br />
proreir. Fu this purpce six lev€ls ofcom saeep tiquo. werc resred viz_, 0.5. t.0,<br />
1.5, 2,0, 2.5 and 3.0 perceni anq selting CN ratjo $ del.min€d pr€viouly. t}le<br />
optunum levet obs€ned durin8 this q\perimcnr was used in $bs€quenr<br />
4.7 Setting Time for Addition ofCSL<br />
Ir wd required to determin€ fie opdmun rime for supptementation of<br />
ophnized lwel of CSL. for riis purpose lh@ rin.s of hcrlbation wcre rcsrcd viz.,<br />
4E. 60 ed 72 hou6. The ft€sk ofalt rlE tine fE.iods we.c narvesrcd at 96 houB of
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