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60<br />
gram ofdn€d biomass was tat€n for estinatior ofru€ prot€in by rcating wilh 30<br />
ml of5% Trichloroaceric acid (TCA) in warer bati ar 90'C rempemrure.<br />
4. Optimization of Fermentation Conditiotrs for<br />
Arschniotus sp.<br />
4.1 Elfect ofType of Rice polishings.<br />
h lhis expcrimenr codpsrison was madc b.rwed undefafi.d rice<br />
polishings and defatled .i@ polishings for ihe producrion of biomals protejn.<br />
Triplicate flasks of€ach type of rice polishings containing 50 mt grcMi medium<br />
were prcpared md incubarcd for 3 days udcr prcdctemined conditioN viz., .ice<br />
polishius 5.00lo, urca 0.3125yo, Caclr 0.0025%. MgSO..THrO 0.0025%, KHrpO.<br />
0.2%. pll4.0 add l€npe.ature lo"C (Mahnood, 1990). The biomass so preparcd<br />
was dried and esrimared for rrue lrolein. nle amount aod B?e of subsrnte used in<br />
Ois qperiment w8 adopted in thc suts.quenr exp€rimenls.<br />
4.2 Effect ofAcid Treatment ofsubstrate<br />
An experiment wa! @nducled usiDg defalM rice polishinSs and fte<br />
ellect ofrrcarme of sqbsrnre wirh 5% Hrsoa @ I mlrg wrs srldied. on the basis<br />
ol reull of rhis cxperimcn! r.€rcd subsrmre w6 used ,n tnc subs.qu.rr<br />
4,3 Addition of Different Levels of Morasses at 4E hours of<br />
F€rmentation.<br />
The effecr of addition of motasses at 48,i hour of Srowth ol<br />
Atachhiot6 tp. was studi€d in this cxperim€ni For lhis purpose 5 leveh of<br />
molassd werc resred viz., l, 2, 3, 4, and 5 p€rc€nl tor lne maxrmuD producrion of<br />
b,omss protein and hawestcd after 72 hous of fcmenration. The opii,rum tcvel of<br />
molass€s was used in rhe subsequ. experin€nts.
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