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4i<br />
markedly inll €nced fic cGcuhurc €quilibrium as well as cell yield, whicl $as<br />
high at higl€r yeost extract level. The yield varied from 0.26 to 0.48 g/9.<br />
Alhar e/ al. (1995) reporred thc specilic grcwln 6lc 0r) 0.180hr, s ofcell<br />
mN fmed ps t of substmre udliT.ed {Y*) 0.49 ed I of prcduci (prolein) fomed<br />
p$ 8 ofcell mas (Yed 0-59, during fenncnration ofbe€t plllp in fed barch culturc<br />
sysrem, first witlf ca"dlda ,rtli' t']Jen wln B.Jlaw.<br />
shojaoradali and Chaali (1996) hol.led twenty colonies of Dethylotophic<br />
b.cEia fioo soil sanples of oil lield dq ed wte yaler ol oil disrilldies for<br />
singl. ceU pmtein ploduction. The conlinuous culrre t@hnique und.r chemctat<br />
qd Bed 10 optimi?e the growrh conditions ofbacrerial culturc CA 16,1. Opimll<br />
param€res such as pH (6.8), l€nperalur€ (3?'C), aemrioD rare (lvol/vollr),<br />
asiralion speed (a0orpm) and dilution rale (0.15h') werc mainrained. The<br />
manmum biomass yield coeflicienl (Y$) oblain€d uder optinizcd condilions was<br />
0.4lgg'-<br />
Vevinit ?r al. (1996) 6ed barch dd conlinuolr cultivarions to invesdgsr.<br />
the single cell prcGin (SCP) prcdn rion by CepJel^potiW ei.hhorni@ 152 in d<br />
air lin rcactor. fie culture medium consisl€d of (sava ss a sole ca$on source,<br />
(Nr!)rSOa 65 a nilroso souc md KHlPOr !,3 a buffcring ag.nt. The pH of rh.<br />
medim w4 adjulted to 3,E. ln batch f.m.niadon l% c,gva &v.1he hiSh.sr<br />
yi.ld of 0.4. The optinum coodition fo. ontinuou fm.ntaion wls I % 6s&va in<br />
feed m€dilrn ar a dihtion 6G of0.4hi.<br />
B. BIOLOGICAL EYALUATION<br />
The cheDical analysis givcs us only infomarion abour rhe presence of<br />
nulrients but it does not lell us about rheh digesiibiliry ud .vailabitiry for thc<br />
biological system. rtis @ only bc jld8ed tlnousb biological rriah. Sisniticanr<br />
available litcElw on the biologiql cvllution of products ohqined s a rcsutr of<br />
femmt lion har been.wiqed wi$ special refeftn@ ro fed conslnltion, wcighr
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