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40<br />
having pll 5.5 whcD femented wilt, LacbbacillB btlgdt !JJ69 fo.24h result€d<br />
in Dqi,rum produclion of lactic acid. Addition of y6r extEct plus MnCl, gave<br />
the highBl production of ladic acid (1.?l g/L/h), sp6ific acriviry of LDH (1.03<br />
U/'r8 prolein) and ScP (32.8%).<br />
K.rthikeyan d dr (1996) oprimized rhc nutienr medium compcirion<br />
(conla'n'ng sucrose, yeasl exkact and K:HPO,, lempc.alure and inilial ptl<br />
conditions for batch dexrr.n producrion in shate flask fementalions using a srain<br />
of LelconNtoc nesenteliodes NRRL B 512 (F). T}'ey rcpon€d rhe optimal valu€s<br />
6 sucrose 3009/1, y6t extact l0g/L, KTHPO4 308/L, rempemture 23 oC ed iniliat<br />
pH 8.1 wirh ipredicred dexhnyietd ot t54 gtL.<br />
Okada and Shingo (1996) compared rwo media fof s€lectiv€ gr0{rl) and<br />
diagnosis of Ba.ir6 .er€rr. They found ttal $e yeasr cxtract subsriurion wirh<br />
mear exkact in O€ cultue medium havjng p€ptonc, NaCl, Stycine. meat exrnct,<br />
laclose, phenol red, polymyxia B suthre, agd a8d .nd ess yotk lsulred in stabt€<br />
dd sup€rior media t@d for $lective gron1h dd diagnosis of A cef.rr<br />
Lo et al. (1997) srudied the synergelic effecrs of ca$on (Slucose) aM<br />
nilrogcD 06t qrract substr.les otr cclt growrh &d brhs producrion. Tle eff€cl<br />
of Slucosdyedl €xrract ratio (C/yE) was studied in veious opemting models<br />
includiog batch, 1*o srage barch 8d fcd brrch f.nnenLtiols. They obs€ryed that<br />
both xdrhe yield .nd specific produdion nte inc@sed wirh ind€asin8 C,fyE in<br />
rhc m€diuD. Bur thc cell yield and spccific grcw$ deoea!€d 6 c,ry[ shitl fron<br />
ard inili.l low lcvcl (2.5% glucose/0.3% yea{ exrncl) ro a tigh tdel (5.0%<br />
sluaosc/0.30l, yeast extocr) ar rhe end of exponcnrial Browth phlse which was<br />
prcfeEble for xanthm pmduction. lr wss also found rhar a ,noderatcly high yeast<br />
extacr concentrar'on in (ne mcdium rcsutred in high cell dcnsily beforc the cutturc<br />
ent€red in slalionary phase.
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