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169<br />
added and lhe volurnes werc mad€ upro lomL with distill.d w.rer (Tabl.-33).<br />
Opricaldensily ofthese solulionswea Deasured at 700nm on speckophotometer.<br />
Tle tesr tubes werc shaken thoroughly and allow€d lo sland for 5<br />
minutes. Optical density was lhen m€asured on spe€lrohpotometer al ?00nm<br />
wavelengih aner sefling zdo .bsorbaDc€ with blank.<br />
Table l 3: &!!83!!e!_etlbe!phea!<br />
Tsr Tubcs Volum€s (mL)<br />
t234561<br />
0.15 % KHTPO. 0_25 0.50 0.7s r.00 r.25 l_50<br />
Amnonium Molybdal€r 1.00 1.00 1.00 1.00 1.00 1.00 1.00<br />
12.s%)<br />
Aminonephthol 0.50 0.50 0.50 0.50 0.50 0.50 0.50<br />
Distiued Water 8.25 8-00 7.75 ?.50 7.25 7.00 8.50<br />
r Dksol\rd 25cin 200 tr,Ldisrilled warq in lL vollmelric fl.sk, plaoed,l00mL loN H,SO.,<br />
,dd.d ro molyfi.t elution.nd dilur.d ro |L silh dhtill.d s.t.r<br />
.r l,ls.d l9tnn of 15% sodiun bisulphit€ solution in a slass sloppecd cylindq !dd.
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