t-,/rrr',r47c

1.6 Nitrogen Fr€c Extr.ct (NFE)
162
ueigllelAL X 100
NFE wa olculatd by ditT.Ene usins $c followirg fomulr
NFEo?6 r 100-(voC.P+o?6E.E+96c.F+o/6Ash)
2. SolubleCarbohydrates
The solublc erbohydrat€s io lhc biolt'ris filtra(c $.!5 estinrr.d by d|c
m€thod of Dubois er al (1955).'ne @nseurivc votllmcs of0.l p€rccnr gtucos.
solution w.E trlt|sferrcd ro rhrc. rcat rub6 (I!btc-l l), wh@!s rhc 4rh rcsr rubc
e.v!d a bldk. All rh. rdr tubc qs trclr.d with lmt @h of 0.25N H2SO. plB
lml of 80% aquous phenol. Finally 5ml c-onccntrared H2SO. yas rdd.d ro .lch Gsr
tubc and mix€d. 'nlc ab6orb6rcc of orangc y.llow colour devclopcd, was mcjsurd
on spc.lropfioaom€lcr at 490 nm. A gnph wss conslrucr.d by ploning rhe
rbsobanc! lgainir conc€ntalion of gtuc{sc, Fig-j.
TrblFl l: Estimnior ofSolubl. Cartlotvdtlre
0. l',t Gluco6.
0.25%H,SO.
0.E5%Phcnol
Distill€d V.tcr
conc. H?SO.
0.1
t.0
t.0
0.9
5.0
Dilutions (mL)
3 4(Bla )
o.z
t.0
I.0
0.6
5.0
Onc nL cich ofbiona$ fitrllrc (€st nlltcrial) wrs diturcd ro t00 mL
usinS dislillcd wrt€r Ocn L0 mI ofthe ditu. dnwn was ttercd wirh rhc r.o8.nrs
.s u!€d in thc clse of sbndlrd cud.. Thcn f.om tlc aranddd curv. rt. ab6o6cncc
of thc r.action mixrurc wa! r6d .nd cx&.polar.d fo. warcr sotubtc clrbohy.tBr.s.
0.3
1.0
t.0
0.7
5.0
t.0
1.0
t.0
5.0