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I6l<br />
isnir.d in lhc numc tumac€ a( 550 oC. Thc wciShr of s5h wa! rcc4rdcd and from<br />
lh. difrcrenc€ in wcidt thc crudc fibrc pcrenrlg. ws @tcutd€d<br />
= Wci.ht ofR.sidu. - r .iqht oftuh X t00<br />
wcidtofseple<br />
1.4 Crude Protein (C.P)<br />
Thc crud. prorein was dcr.mind following Kjctdlht.s m€dnd. A<br />
lnow|l qu.entily of srinplc rrs digestcd wilh conclnltGd sun ric acid in rl,.<br />
pier€ncc ofdigestion mixrutr m aining KrSO{ CuSOa and F.SOi innrioof100:<br />
l0 : 5. The digcsrcd sampl. yes diht€d upro 250rnl votumc wirh disri cd *iter.<br />
Tcn ml of thh digc,sr.d s$pte plus l0 ml of 40% N.OH was transtdr€d b<br />
MicroKj.ldhat dislillalion applrrtus. Thc mi)(furc was distill.d wili srcEm and rhc<br />
disrilhlc wa! collccrcd in a conicrl flrsk (l00ml) having l0 ml of 2yo boric acid<br />
solution and mcrhyl nd indic{or. Thc conr.r s of r}e ll.sk lr€r. titrar.d aSainsr<br />
standafd N/10 HISO. ro lighr pir* color md poinr. The oirogcn and ,rorcin<br />
conlcors w.Ic catculdcd using lhe fo owins formula.<br />
1.5 tuh<br />
Nitrog€n % =<br />
Protcin pcrc.naaSe =<br />
vol. ofN/l0<br />
Vol. of digcsld sffnplc<br />
lrl(.n for disriuarioo<br />
% NitroSq X 6.25<br />
Wcielt ofsanplc<br />
X talen<br />
A rno$n quantity ofsrhptc was r.sten in cruciblc,nd rlcn igniGd on<br />
oxidizing flsm. of bumcr, till tro srnok. was Sivcn our rhcn ir wrs ignir.d in a<br />
Mumc tumac. at 600oC rcmpcErurc rill .ll rh. o.glnic mrng wa! oxidiz.d. Thc<br />
weight ofash w8s rccordd and rh.n ash per@nl.a8c w!! cltcutarcd uling fotto\|linS
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