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160<br />
Analytical Methods<br />
l. Proximate Antlysis<br />
AO C (1990) nethods.<br />
1.1 Moisture<br />
Th. prorimate nnalysis of subsrrate ud biomsss wa! done fo owins<br />
For noisture esrimalion sampts w.€ w.iShed .rd rhcn dricd<br />
oveD al ?0"C dll consbnl wcishr. Weisht of dried samptes ws rccoded and<br />
the direrencc in initial dd final weighl lhe moisrurc conrcnrs we.c esrrmared<br />
following fomula<br />
Moisturc'/o =<br />
1.2 Ether Extract (E.E)<br />
Inidal Wei !:Einal wei<br />
Inilial WeiShl<br />
!x 100<br />
A known weiShi of $e s.nple es d(en in fiimbl. ard then<br />
cxhcled wirh dierhyl elher in Soxnlefs appaBtus. Aft€r 5_6 exrmc ons the clh€r<br />
ext"ct wd tat.n in p€rri dishes rhen pul in oven for drying fi conjtant wei8ht ar<br />
?0"C t€mperanrr.. The p€rccnr .tft.r exrract ws calcutared by using following<br />
1.3 Crude Fibr€ (C.F)<br />
Eth.r Exkact o/o<br />
A klo*n quartiry ofdried and far tile sarnpl. wss rakell snd refluxed<br />
li6l *ith L25 per@n( sutfuric acid fo. half sn hour, &ercalt€r it was fijrecd md<br />
$orouslly warhed wili disritt€d wale. and rhcn rcnuxed again now wnh 1.25<br />
percot sodium hydroxid€ for hatfan hour. .nE suspcnsio, was fitrercd and Siven<br />
lhorcu8h washing wirb dki €d water. The r$idue was dncd in oven ar 70.C<br />
lenpedtu.e rill coNtant weight Tte weighr of dried r€sidue was rcco.ded and rhcn<br />
using<br />
Weilht of Etb Extracl X 100
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