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99<br />
averae. true protein valu4 of the biomas hsd.sled at thesc rime periods de<br />
Fsenr.d in Tablc 6. The odysis of aridcc of &ra (App.ndix XIX) rcvated<br />
non siSrili@l diffcr€nce bctwa rhe tue prolein values of biomass han€sred ar<br />
24. 48 and 72 houls. This indicalcd rhat mdimum fernentarion r@k plac. llpro 24<br />
houF rlg which il rehained in statidary phe till 72 hou6.<br />
Tle incubalion period oflhc o.ganism deDcnds on i1s kiid. Bacl€ria<br />
ulually rqke short p..iod for mnplelior of rheh fem. ltion_ The incubaliot<br />
period fou d ollimum for fermentdion of ala",l (bacreria) in pEseni study was<br />
24 hours. Molina rl a/. (1984) and Elsabaeny and H6se (1996) also reponed 24<br />
hom incubation p.riod d th. m.ximum for producrion of fcrmeotlrion producrs<br />
for diffcrenl shains of bacleri! in the shak€ @ndition. Using same organism as in<br />
case ofMolina.r di. (1984), Enriqu€z and Rodriqucz (1983) however, obsd€d 48<br />
hoG Lmentation as optimum fd maximllln yield of bactc.ial biona$ of atkati<br />
l.eated bag6s. with C.//!/ohonar lP, This vaiation .d be aitibutcd to rhc kind<br />
of $bsll:te ferm.nted. The rBulls iodicaled 24 hours itrcubario pdiod d rh.<br />
optinum and was lhercfore us.d in funh€r studies.