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92<br />
B. TO OPTIMIZE THE GROWTH CONDITIONS<br />
FOR Brevibacterium lavzz USING FIRST<br />
PHASE PRODUCE BIOMASS AS<br />
SUBSTRATE.<br />
The biomass prQared thrcu8h femenlation of rice pothhinSs *ilh<br />
Arachniotu species udet $eviously oprimized condirions was funh.r femenred<br />
wih A./zy@ with th. t.lset ro achicvc improyed pror.in yietd p.diculdly irs<br />
enriclmlct with lyside.<br />
Objectives:<br />
i) \llh.thct R$@M q g.ow in presence of,tfa.rziona spe.r.r or not for<br />
funis prciein.trrichmdt.<br />
ii) To se€ if the addilion of molass€s, glucN or 8un, com sl.cp liquor and<br />
yeast sludge a1 diflercnl levels and tine intervals ro rhe fermenrarion<br />
medium de ne.€ssary for improving rte protein biomN yield.<br />
iii) To eslablish the oplimum harystinS time for mdimum producrion of<br />
bionrass lrctein.<br />
Results and Discussion:<br />
The results obtained regarding the optimiafon of conditions for<br />
fef,ncnraiion ee discusred c under. Thc biomss prorein coorsls obkined lndd<br />
difiererl conditions acsbown iD Tablc6.<br />
l. Btevlbqclerium ldtaft Culture Cultivrtion<br />
Rteyibrctetiu IM is known d a tysin. synrhsizing bacraia dd<br />
was cultivated on lh€ prc-fennenred m.dium aner adjuring i.s pH ar 7.0. Thc<br />
fennenlation was cani€d ou1 fo.72 houls. Inirially the f€fln€ntarion was canied out<br />
lo see if fie lwo organbm! can grow in syne€isn. 11'€ biomass thus obrained war<br />
hades(.d and analyz€d for ils truc protein clnrenrs (Table 6). Th€ data wer€<br />
subjected to slatistiel analysk ed the analysis ofvadance ofthc dara is shoM in<br />
Appendix XIV. Significant differenc€ (P
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