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Overview for A- art ofCha<br />
'fte defancd rice polishings gave comparabte prorein yield wili undefar€d.<br />
Ior oprimizing filnler femctrr4ion conditio$ defalred ricc polishinSs was<br />
Tle pretreahenr of substrate wirh acid did nor cause significanl<br />
imp.ovemcnr in prot€in yield, so h wd not uscd ir tunh.r rrials.<br />
The addilion ofmola3s.s at lyo lev.l ar 48d hour of fmenhion did nol<br />
Sive any signiticanl ifrprovement in protein yietd sd ws nolsupptemenGd<br />
ro the glowrh medium du.ing n.xt rriats.<br />
The addilion of com steep ljquof (CSL) ar 0.5% l€vel at 48,i hou of<br />
fementation also did not give significa improvemenr in prot€in yield.<br />
Howeler, the improvemenr in this case was bet|er as 6mpe@ ro mojsses<br />
supplem€nhtion- It wa! fierefore adopred during $e nexl datr.<br />
Carbon to nit oSen mrio of l0 : I gave significrnt impruvm.n ,n pole tr<br />
yield and was adopted durin8 rhc next tdals.<br />
Delaying of hNeslirs ft)e above 72 holrs did not give sjgnitior<br />
imprcvem.nt in prolein yield. The hadering rime of 72 hou6 was thercforc<br />
odoprcd becaus€ funhcr delay was unc@nomical.
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