stakeholder panel on infant formula & adult nutritionals (spifan)

VITA‐08 An interlaboratory‐verified
method for the determination of
vitamins A and E in milk‐ and soy‐
based infant formula by LC with
matrix solid phase dispersion
extraction
Chase, WG, Ye, L, Stoakes, VC,
Eitenmiller, RR, & Long, AR (2004) J
AOAC Int. 87(5) 1173‐1178
VITA‐09 Determination of Vitamins A
(retinol) and E (alpha‐tocopherol in
foods by liquid chromatography:
collaborative study
VITA‐10 Foodstuffs – Determination of
vitamin A by high performance
liquid chromatography Part 1:
Measurement of all‐trans‐retinol
and 13‐cis‐retinol Submitted by:
Güenther Raffler
VITA‐11 Foodstuffs – Determination of
vitamin A by high performance
liquid chromatography Part 2:
Measurement of ß‐carotene
Submitted by: Guenther Raffler
VITA‐12 Simultaneous Determination of 13‐
CIS and All‐Trans Vitamin A
Palmitate and Alpha Vitamin E
Acetate by HPLC and Column
Switching: Submitted by Abbott
VITA‐13 Determination of Vitamin A in
Infant/Adult Formula by HPLC‐
Isotope Dilution Mass
Spectrometry
VITA‐14 Determination of Vitamins A
(Retinol) and E (alpha‐Tocopherol)
in foods by LC: Collaborative Study
2001.13
VITA‐15 Determination of Vitamin A
(retinol, vitamin E (tocopherol),
and B‐carotene in food stuff by
HPLC‐UV
CHAIR
RECOMMEND
CHAIR
RECOMMEND
CALL FOR
METHODS
CALL FOR
METHODS
CALL FOR
METHODS
CHAIR
RECOMMEND
SMPR
Table 2 ‐ Candidate Methods Recommended by Working Group
Recovery data for IF. 2004 Matrix solid‐phase
dispersion; evaporation;
filtration; normal‐phase LC ;
fluorescent detector.
Recovery data for IF. Use of
ZCM. 2002
No data on IF. Precision and
Reproducibility data for
butter and milk powder.
2000
For β‐carotene; SPIFAN WG
decided not to include β‐
carotene in its scope. 2000
Good precision and recovery
data for IF.
Uncertainty, expanded
uncertainty, and trueness
data using NIST 1849.
LOD, accuracy, and expanded
uncertainty, trueness data.
Some IF data.
Saponification w/ ethanol‐
water; LC; UV detector.
Saponification by alcoholic
KOH; extraction w/ diethyl
ether; HPLC; UV or
fluorescent detector.
Saponification by alcoholic
KOH; extraction w/ diethyl
ether or acetonitrile; HPLC;.
Methanol extraction;
centrifugal separation; HPLC
w/UV detector.
Saponification w/pyrogallol
and KOH; extraction w/
petroleum ether; filtered
through Na SO4; HPLC ‐
isotope dilution mass spec
[HPLC‐IDMS].
Saponification w/ KOH;
extraction w/ petroleum
ether; HPLC ‐ UV/VIS
File No. Manuscript Title & Publication Information Working Group Comments
VITE‐01 Vitamins A and E by UPLC‐UV or FLD submitted by Campos‐
Gimenez
VITE‐09 Determination of Vitamins A (retinol) and E (alpha‐tocopherol in
foods by liquid chromatography: collaborative study
Well written, recently developed method. Request data.
Undated: current.
Recovery data for IF. Use of ZCM. 2002
Vitamin E Working Group Meeting Minutes PRE‐DECISIONAL ‐ DO NOT DISTRIBUTE
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