stakeholder panel on infant formula & adult nutritionals (spifan)

Table 1‐ Proposed Methods
VITAMIN A METHODS
File No. MANUSCRIPT TITLE AND
PUBLICATION INFORMATION
VITA‐01 Vitamins A and E by UPLC‐UV or
FLD submitted by Campos‐
Gimenez
VITA‐02 Immunoassay Method for
Determination of Vitamin A in
Infant Formula Submitted by:
SciMed Technologies Inc.
VITA‐03 Off‐line coupling of pressurized
liquid extraction and LC/ED for the
determination of retinyl acetate
and tocopherol in infant formula
Delgado‐Aamarreno, Bustamante‐
Rangel, M, Garcia‐Jimenez, M.
Sanchez‐Perez, A, & Carabias‐
Martinez, R. (2006) Talanta 70,
1094‐1099
VITA‐04 Simultaneous analysis of Vitamins
A and E in infant milk‐based
formulae by normal phase HPLC
DAD using a short narrow‐bore
column Chavez‐Servin, JL,
Castellote, AI, Lopez‐Sabater, MC
(2006) J. Chormatogr A 1122, 138‐
143
VITA‐05 Rapid determination by reversed‐
phase HPLC of Vitamins A and E in
infant formulas Rodas Mendoza,
B., Morera Pons, S, Castellote
Bargallo, AI, & Lopez‐Sabater, MC
(2003) J Chromatogr A 1018, 197‐
202
VITA‐06 Development of methods for the
determination of vitamins A, E and
beta‐carotene in processed foods
based on supercritical fluid
extraction: a collaborative study
Mathiasson, L, Turner, C., Berg, H.,
Dahlberg, L., Theobald, A., Anklam,
E., Ginn, R., Sharman, M., Ulberth,
F., & Gabernig, R. (2002) Food Add
& Cont. 19(7) 632‐646
VITA‐07 Interlaboratory verified liquid
chromatographic method for
analysis of Vitamins A and E in Soy
Based Infant Formula Powder
Chase, WG, Ye, L, Stoakes, VC,
Eitenmiller, RR, & Long, AR (2004)
87(6) 1329‐1333
Source Comments method description Rank*
CALL FOR
METHODS
CALL FOR
METHODS
CHAIR
RECOMMEND
CHAIR
RECOMMEND
CHAIR
RECOMMEND
CHAIR
RECOMMEND
CHAIR
RECOMMEND
Well written, recently
developed method. Request
data. Undated: current.
Some repeatability data
(misidentified as
reproducibility) for IF. Good
% CV. Not sure if method can
detect at proposed LOQ.
Undated: current.
Some recovery data for
retinyl acetate in IF. 2006
Good LOQ; recovery; and
precision data. 2006
Precision. sensitivity,
linearity, and recovery data
for IF. 2003
Precision, reproducibility, and
recovery data for IF. 2002
Alcoholic saponifcation; SPE 3
w/ diatomaceous earth;
evaporation; HILIC‐UPCL or
UV
ELISA 2
Methanol extraction;
pressurize liquid extraction
(PLE); saponification with
KOH; liquid chromatography.
Ethanol extraction, hexane
re‐extraction; HPLC‐diode
array (DAD).
Ethanol extraction, hexane
re‐extraction; reverse‐phase
HPLC; photo diode array.
Ethanol saponification;
supercritical fluid extraction;;
HPLC; UV or fluorescence
detection
Recovery data for IF. 2004 Dehydration w/ MgSO 4;
extracted w/ isopropanol and
hexane‐ethyl acetate;
evaporated, filtered; LC; with
fluorescence detector.
Vitamin E Working Group Meeting Minutes PRE‐DECISIONAL ‐ DO NOT DISTRIBUTE
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