Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

immunosorbent assay) and RT-PCR (reverse-transcription polymerase chain reaction. No
virus was isolated in the isolation studies. The supernatants from passage and isolation
samples were checked nucleic acid and viral antigen of VHSV by RT-PCR and Ag-ELISA.
Neither nucleic acid nor viral antigen was detected from cell culture supernatants and
tissue extracts.
KEY WORDS: Rainbow trout. Viral haemorrhagic septicemia. RT-PCR. Ag-ELISA. CPE
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