Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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�� edilebilmektedir. Bu yöntem ile konsantrasyonda virus süspansiyonu içerisinde bulunan �� ölçüde giderilmektedir. �� lde PEG 6000 ilave edilir. �� o C’de 2 saat bekletilir. Takiben 2500 rpm de 40 �� -0,1 M Tris tamponu pH=7,8 ile resüspanse edilir. Resüspansiyon hacmi sonuç istenen hacim ve �� [48]. 2.6. 146 S D��Belirlenmesi: �� o C’de 41000 devirde 2 saat ultrasantrifüjde santrifüj etmektir. Bu amaçla 0,15 M NaCl-0,1 M Tris tamponu (pH=7,8) ile stok çözeltisi �� Santrifüj tüpünd�� rutin çal�� verilmekte böyl�� ap edilir [Barteling, S.J., ve Meloen, R.H., 1974, Allington, R.W., ve ark., 1976, Doel, T.R., ve ark., 1982]. 328
�� Konsantre edildikten sonra 146S testi ile elde edilen absorbans pikleri.Örnekler 40 defa seyreltildikten sonra test edilmi��. 2.7.OVK-OVA PEK Sistemleri T��Tutukland�� o �� -480 µg/mL 146S �� -OVA �� -��Bunun da �� o �� ± �� OVK-��ü�� sonra santrifüjlenen örneklerin ü�� testi sonucunu gösteren örnek grafik. 329
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Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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