Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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�� OLU�� ÖNSÖZ �� - poliovirus tip 3 D antijenine �� –�� noklonal Antikor Panelleri Üretilmesi �� ÖZ �� -c farelerden 9 �� ine dirençli ve sürekli olmayan bir �� 300
�� te edilebilir. Anahtar Kelimeler:�� ABSTRACT Nine Foot and Mouth Disease Virus non-nötralizan monoclonal antibodies were produced from Balb-c mice immunized with FMDV (Foot and Mouth Disease Virus) type A (A�� FMDV, suggesting that the binding epitopes of the nine Mabs are conserved between subtypes A. These Mabs are contain IgG1 and IgG2a isotypes. In order to define the Mab binding epitopes, the reactivity of these Mabs against trypsin-treated and denatured FMDVwere examined using an indirect ELISA. These Mabs recognized an epitope which is trypsin resistant and discontinuous. These Mabs binding to FMDV are dependent on conformational structures of intact viral particle, since it failed to recognize any viral protein in Western Blot. Because the use of Mabs increases the specifity, accuracy and efficiency of diagnostic tests compared to polyclonal antisera, these nine Mabs are suitable for type A dependent diagnosis of FMDV, such as DAS ELISA or could be adapted to rapid test. Keywords: Monoclonal Antibody, Foot-and-Mouth Disease Virus, Epitope �� enik özelliklerinin belirlenmesi, �� -�� önemlidir. Türkiye’de 19�� Türkiye’de 1997-�� 301
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10. OIE -Bovin Tuberculosis Chapter
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13. KAUFMANN, S.H.E., Van Embden, J
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ovine Virus diarrhea virusu (BVDV),
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1997; Petrovec ve ark., 1999; Aleks
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Denmark, H.A., Stanford, M.T. 2000
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SUMMARY: The detection and inhibito
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environment they live in, or during
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Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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