Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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,Stabilite
SUMMARY
Stability studies on 146S particles of inactivated and concentrated FMD virusantigens
The aim of this study is to evaluate the effect of non-specific proteins which were
concentrated together with virus inactivated by BEI and concentrated by cross-flow
method on 146S amount during storage at 4 ºC and -20 ºC.
At 12th month of study, it was detected 10 % of antigen loss amount of 146 S in
consantrated O type vaccine antigens stored at 4 o C expecially in group 3 and 4 (Group 3:
Inactivated antigen 50% + Dulbecco PBS 48.5%+ Chloroform 3% + NaHCO3 1% Group
4: Inactivated antigen 50% + Dulbecco PBS 48.5%+ Chloroform 0.5% + NaHCO3 1%)
and 30 % of antigen loss was detected in group 6 which was control group.
All groups of consantrated A type vaccine antigens stored at 4 o C, after 3th month,
it was seemed 15 % of antigen loss amount of 146 S and this loss reached %90 at 12th
month. 95 % of antigen loss was detected in group 6 which was control group of A type
antigens. But group 3 and 4 was showed slightly higher stablity than others which have 60-
70 % of antigen loss amount of 146 S at 6th month.
For all study groupsof both type of antigens that stored -20 ºC, Although a sudden
decline has been detected in all groups at 3th month , relatively more stability have been
detected for group 3, 4 and 6th.
It was detected that % 30 of loss amount of total protein in all groups of both type
antijgen stored 4 o C and % 60 of loss amount of total protein was detected those stored at -
20 o C.As a result, although antigens were more stable at 4 ºC than -20 ºC, it was thought
that -70 ºC and if itis possible -196 ºC liquid nitrogen would be better for antigens storage.
However, because of the volume problems that caused by large scale production at this
time, it isn’t possible that storage of the consantred antigens in -70 ºC and -����������������
case, antigen preparations described in group 3 and 4 will be more useful for routine use
Recently,in fmd instutute,although concentrated antigens are purified by using
PEG to get rid of non-specific proteins, it was thought concentrated antigens may be
stored at 4 o C up to two year by using further purification tecniques.
Key words: Inactivated fmdv antigens, Concentrated fmdv, Chloroform, 146 S , Stability
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