Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

More documents

Recommendations

Info

�� o �� birlikte 3. ve 4. gruplarda 6. aydan sonra % 60-�� -20 o C �� o C’ de ortalama % 30; -20 o �� Sonuç olarak 4 o �� -20 o C’ye göre çok daha iyi ��-70 o C ve/veya -196 o �� antijenlerin daha stabil ola�� -70 o C ve/veya -196 o C’de antijenlerin �� o �� o �� ABSTRACT Stability studies on 146S particles of inactivated and concentrated FMD virus antigens The aim of this study is to evaluate the effect of non-specific proteins which were concentrated together with virus inactivated by BEI and concentrated by cross-flow method on 146S amount during storage at 4 ºC and -20 ºC. At 12th month of study, 10 % antigen decrease was detected in concentrated type O vaccines stored at 4 o C, especially in group 3 and 4 (Group 3: Inactivated antigen 50% + Dulbecco PBS 48.5%+ Chloroform 3% + NaHCO3 1% Group 4: Inactivated antigen 50% + Dulbecco PBS 48.5%+ Chloroform 0.5% + NaHCO3 1%) and 30 % of antigen decrease was detected in group 6 which was control group. All groups of concentrated type A vaccine antigens stored at 4 o C, after 3th month, 15 and 90 % of antigen decreases were detected after month 3 and at 12th month respectiveley. 95 % of antigen decrease was detected in group 6 which was control group of type A antigens. However group 3 and 4 showed slightly higher stability than others which showed 60-70 % of antigen decrease at month 6. For all study groups of both type of antigens that stored -20 ºC, a sudden decline has been detected at month 3. For group 3, 4 and 6, relatively more stability have been detected. Thirty percent decrease for those stored at 4 C and 60 % for those stored at -20 C were observed in total protein levels of all groups of both antigen types. Overall, antigens stored at 4 ºC were more stable than those stored at -20 ºC. Of course -70 ºC and -196 ºC would be better for antigens storage. Although we did not try them in this study because of the volume problems that caused by large scale production. In this case, antigen preparations described in group 3 and 4 will be more useful for routine use. Today concentrated antigens are purified using PEG to get rid of irrelevant proteins. It was suggested that concentrated antigens may be stored at 4 o C up to two year by using further purification techniques. Key words: Inactivated fmdv antigens, Concentrated fmdv, Chloroform, 146 S , Stability 282
�� -100 dür. Mortalitesi ise genç hayvanlarda akut ��-70) olmakla beraber erginlerde ancak % 2-5 civar�� ölümlerde bildirilmektedir (7 ). �� getirilir (1). Etken 23 nm. büyüklükte olup tek iplikçikli RNA içermektedir. Kübik �� 7 serotipi ( A, O, C, ASIA, SAT-1, SAT-2, SAT-3 ) ve bu tiplerin en az 60 kadar alt tipleri �� lizleri epidemiyolojik �� o C nin üzerinde �� olarak in�� nakledilirler (4). �� uygulanma�� A�� söz konusu �� olarak inaktive edilmesinden sonra aluminyum hidroksit [Al(OH)3�� -flow teknolojisi virus konsantrasyonu iç��- flow filtre sistemi, virusu �� -�� adet protein (VP1, VP2, VP3,�� adsorbsiyonu ve antijenik ö�� 141-�� -spesifik proteinler, �� 283
Page 1 and 2:
T.C. ��
Page 3 and 4:
��
Page 5 and 6:
��
Page 7 and 8:
59 ��
Page 9 and 10:
TC ��
Page 11 and 12:
��
Page 13 and 14:
��
Page 15 and 16:
10. OIE -Bovin Tuberculosis Chapter
Page 17 and 18:
7. ��
Page 19 and 20:
��
Page 21 and 22:
13. KAUFMANN, S.H.E., Van Embden, J
Page 23 and 24:
ovine Virus diarrhea virusu (BVDV),
Page 25 and 26:
��
Page 27 and 28:
YILLARA GÖRE ��
Page 29 and 30:
34. ��
Page 31 and 32:
(3) ve Özkul ve ark. (27)� �
Page 33 and 34:
�� : SDS-PAGE
Page 35 and 36:
��
Page 37 and 38:
��
Page 39 and 40:
7-Bishop R.F., Hewstone A.S., David
Page 41 and 42:
TC ��
Page 43 and 44:
1997; Petrovec ve ark., 1999; Aleks
Page 45 and 46:
��
Page 47 and 48:
��
Page 49 and 50:
28-Randall, K., Saiki, B.S., Chu-An
Page 51 and 52:
��
Page 53 and 54:
��
Page 55 and 56:
immuncomb(IC)(9), direk agglutinasy
Page 57 and 58:
TC ��
Page 59 and 60:
��
Page 61 and 62:
5- Aslan V., Tiftik A. M. (1987). 1
Page 63 and 64:
TC ��
Page 65 and 66:
��
Page 67 and 68:
��
Page 69 and 70:
TC ��
Page 71 and 72:
vi) Öte yandan patojen E. coli�
Page 73 and 74:
��
Page 75 and 76:
��
Page 77 and 78:
(verotoxin)-producing Escherichia c
Page 79 and 80:
TC ��
Page 81 and 82:
��
Page 83 and 84:
��
Page 85 and 86:
MS-Primer R (20 pmol/ µl) : 1 µl
Page 87 and 88:
��
Page 89 and 90:
36. Sanei B, Barnes H. J., Vaillanc
Page 91 and 92:
��
Page 93 and 94:
��
Page 95 and 96:
7. Gürtürk K, Ekin I.H, Aksakal A
Page 97 and 98:
��
Page 99 and 100:
Nazal ve konjunktival swap örnekle
Page 101 and 102:
��
Page 103 and 104:
��
Page 105 and 106:
Test samples will be collected rand
Page 107 and 108:
��
Page 109 and 110:
��
Page 111 and 112:
61.8°C’ de 30��
Page 113 and 114:
��
Page 115 and 116:
ve Anti��
Page 117 and 118:
TC ��
Page 119 and 120:
��
Page 121 and 122:
4- Bakonyi, T., Derakhshifar, I., G
Page 123 and 124:
��
Page 125 and 126:
8.3. Materyal ve Metot: 8.3.1 Mater
Page 127 and 128:
��
Page 129 and 130:
TC ��
Page 131 and 132:
Denmark, H.A., Stanford, M.T. 2000
Page 133 and 134:
��
Page 135 and 136:
��
Page 137 and 138:
��
Page 139 and 140:
�� GÜNGÖ
Page 141 and 142:
17. MURPHY, F. A., GIBBS, J. E. P.,
Page 143 and 144:
SUMMARY: The detection and inhibito
Page 145 and 146:
��
Page 147 and 148:
Aprotinin; Serin proteaz tipi enzim
Page 149 and 150:
1. Doel, T. R., Collen, T. (1982):
Page 151 and 152:
environment they live in, or during
Page 153 and 154:
Ayraçlar: ��
Page 155 and 156:
��
Page 157 and 158:
10.2. ��
Page 159 and 160:
TC ��
Page 161 and 162:
��
Page 163 and 164:
Konjugat:� ��
Page 165 and 166:
6. Grotmol S., Todland G.K., (2000)
Page 167 and 168:
TC ��
Page 169 and 170:
��
Page 171 and 172:
��
Page 173 and 174:
��
Page 175 and 176:
25. Sitjà-Bobadilla, A., Alvarez-P
Page 177 and 178:
��
Page 179 and 180:
��
Page 181 and 182:
��
Page 183 and 184:
TC ��
Page 185 and 186:
��
Page 187 and 188:
��
Page 189 and 190:
12. ��
Page 191 and 192:
TC ��
Page 193 and 194:
8.2. Literatür Özeti: ��
Page 195 and 196:
��
Page 197 and 198:
��
Page 199 and 200:
��
Page 201 and 202:
ve mortaliteleri ile bunlar�� e
Page 203 and 204:
��
Page 205 and 206:
��
Page 207 and 208:
��
Page 209 and 210:
��
Page 211 and 212:
��
Page 213 and 214:
��
Page 215 and 216:
Tablo 18 ��
Page 217 and 218:
��
Page 219 and 220:
��
Page 221 and 222:
��
Page 223 and 224:
��
Page 225 and 226:
��
Page 227 and 228:
��
Page 229 and 230:
��
Page 231 and 232: ��
Page 233 and 234: ��
Page 235 and 236: ��
Page 237 and 238: ��
Page 239 and 240: sonucunda 8’inde (%8) P.larvae�
Page 241 and 242: 14. Dobbelaere, W.D., Graaf de, D.C
Page 243 and 244: amounts were prepared with current
Page 245 and 246: ��
Page 247 and 248: ��
Page 249 and 250: 3.4.5. Sterilite ��
Page 251 and 252: ��
Page 253 and 254: Log ELISA 3 2.5 2 1.5 1 0.5 0 1 3 5
Page 255 and 256: ��
Page 257 and 258: Tablo.9. ��
Page 259 and 260: Tablo.12. POST-MORTEM MUAYENE PD50
Page 261 and 262: ��
Page 263 and 264: ��
Page 265 and 266: Hamblin, C., Kitching, R.,P., Donal
Page 267 and 268: adaptation period growth kinetics a
Page 269 and 270: hücrenin serumsuz ortamda üretili
Page 271 and 272: 6M (%2SBP): % 2 soybean pepton, tra
Page 273 and 274: ��
Page 275 and 276: Çizelge.1. ��
Page 277 and 278: ��
Page 279 and 280: Öneriler;��
Page 281: ��
Page 285 and 286: ��
Page 287 and 288: Grafik-2 SAV-��
Page 289 and 290: ��
Page 291 and 292: ��
Page 293 and 294: Grafik-8 SAV-21 Konsantre antijenin
Page 295 and 296: ��
Page 297 and 298: ��
Page 299 and 300: 22. Lombard M, Füssel AE. (2007):
Page 301 and 302: ��
Page 303 and 304: ��
Page 305 and 306: ��
Page 307 and 308: Deney hayvanlar��
Page 309 and 310: ��
Page 311 and 312: ��
Page 313 and 314: Grafik 4-��
Page 315 and 316: ��
Page 317 and 318: 18-��
Page 319 and 320: ��
Page 321 and 322: ��
Page 323 and 324: ��
Page 325 and 326: ��
Page 327 and 328: Toplam BSA kütlesi = film kütlesi
Page 329 and 330: �� Konsantre
Page 331 and 332: 20. Aucouturier, J., Dupuis, L., Ga
Page 333 and 334:
��
Page 335 and 336:
��
Page 337 and 338:
3.2. Metot 3.2.1. Örneklerin Homoj
Page 339 and 340:
��
Page 341 and 342:
18.HOPPER, K., (1989). The isolatio
Page 343 and 344:
A SINIFI��
Page 345 and 346:
��
Page 347 and 348:
��
Page 349 and 350:
3.2. Metot ��
Page 351 and 352:
Tablo 4��
Page 353 and 354:
Summary Listeria monocytogenes was
Page 355 and 356:
��
Page 357 and 358:
��
Page 359 and 360:
��
Page 361 and 362:
��
Page 363 and 364:
Yeast extract......................
Page 365 and 366:
��
Page 367 and 368:
��
Page 369 and 370:
Resim 3.3.1. ��
Page 371 and 372:
��
Page 373 and 374:
46. Unestam, T., Weiss, D. W. (1970
Page 375 and 376:
��
Page 377 and 378:
��
Page 379 and 380:
��
Page 381 and 382:
��
Page 383 and 384:
��
Page 385 and 386:
��
Page 387 and 388:
��
Page 389 and 390:
��
Page 391 and 392:
��
Page 393 and 394:
��
Page 395 and 396:
��
Page 397 and 398:
��
Page 399 and 400:
��
Page 401 and 402:
��
Page 403 and 404:
��
Page 405 and 406:
��
Page 407 and 408:
��
Page 409 and 410:
��
Page 411 and 412:
��
Page 413 and 414:
500 bp MW 1 2 3 4 5 6 7 ��
Page 415 and 416:
��
Page 417 and 418:
��
Page 419 and 420:
8.2.Proje Faaliyet Takvimi: ��
Page 421:
49. Dr. Gülhan AYNAGÖZ ��
show all

Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

Create successful ePaper yourself

Delete template?

Save as template?