Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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�� Abstract Mycobacterium bovis has a similar genome construction (99%) with Mycobacterium tuberculosis and it is one of the tuberculosis agents which constitute the “consumption” as the disease is known in public. It has preserved its characteristic for years and it has caused disease in people whose occupation is stoockbreeding. The disease still exists in our country and it is present in the list of diseases with obligatory denunciation. Organizations such as WHO (World Health Organization), IUATLD (International Tuberculosis and Lung Disases Organization) and CDC (Control Disases Centre, Atlanta, USA) are deeply involved in research to increase the knowledge of epidemiology of tuberculosis and they support this kind of research. The eradication of the disease is reported in some countries in the European continent (Denmark, Czech republic, Norway, Sweden, Estonia, Island, Cyprus). To enable the export of milk and milk pruducts from Turkey to E.U countries, establsihing disease-free farms has been decided on. The Ministry of Agriculture and Rural Affairs has done studies in �� provinces for this purpose. In these reports, 150 tuberculosis events were reported to occur in 2005, tuberculin tests were performed in 5976 cows except disease-free enterprises and the prevalance was found to be 10 %. This project aims the isolation and genotyping of Mycobacterium bovis in the bovine internal organ samples which tuberculosis was diagnosed in the sla�� Veterinary Control and Research Institute and Agean University Faculty of Medicine’s Department of Microbiology and Clinical Microbiology. The project is planned in two sections. First of all, internal organ samples from cows macroscapically diagnosed as �� provinces, will be obtained and transffered to the Mycobacterium laboratory of the Microbiology and Clinical Microbiology Department in Faculty of Medicine of Ege Universty. The second section will consist of the laboratory work in which the isolation, identification, antibiotic sensitivity profile determination and genotyping from the samples will be carried out. �� Mycobacterium bovis, spoligotiplendirme, PCR, MIRU- VNTR (Mycobacterial interspersed repetitive units- variable number tandem repeats), �� Mycobacterium bovis, spoligotyping, PCR, MIRU-VNTR (Mycobacterial interspersed repetitive units- variable number tandem repeats), genotyping, antibiotic sensitivity profile, bovine bacteriology. 8. �� 8.1 �� 1. Proje �� Mycobacterium bovis’in mikrobiyolojik ve �� bölgeye ait genotipik verileri elde edilecektir. 2. �� M. bovis� �� profilleri tespit edilecektir. 3��obiyoloji Ana �� identifikasyonunu yapabilir duruma getirilmesi konusunda laboratuar deneyimi 10
�� 8.2 Literatür Özeti: OIE (Office International des Epizooties)’ye göre B listesinde yer almakta olan �� lmektedir. �� ehir ve Konya illerinde �� Tüberkülozun kesin �� M.bovis infeksiyonunun� �� dan elde edilen verilerdir. OIE �� 3, 4). 8.3. Materyal ve Metot: Materyal ��ükleri �� Kesimevi’nde �� veteriner hekimlerince �� - 2007- 2008 ��-�� Fakültesi Mikrobiyoloji ve Klinik Mi�� 11
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Hayvan Sağlığı Araştırmaları 2009 Yılı Program - Tagem

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