download/files/LCQ Deca_Duo Hardware Troubleshooting_8601.pdf

LCQ Deca/Duo Hardware
Troubleshooting
Ed Gonzalez
Product Support

Presentation Topics
◗ Overview of the LCQ Ion Optic System
◗ Tour of the LCQ Ion Optic System
– Making Ions
– Transferring Ions
– Filtering Ions (Quadrupole Ion Trap)
– Detecting Ions
◗ LCQ Maintenance
◗ LCQ Diagnostic Overview
◗ Front Panel LEDs Indications

Mass Spectrometer
Simplified Schematic
Sample
In
Make
Ions
Transfer
Ions
Filter
Ions
Ion Optics System
Detect
Ions
Data
Out

200,000
15,000
1,000
Molecular
Weight
Choice in Making Ions
GC
Non Polar
Polarity vs. Molecular Weight
APCI
PBI
ESI
TSP
FAB
Polar

Simplified LCQ Schematic
Sheath Liquid
Auxiliary Gas
Sheath Gas
ESI Needle Assembly
Heated Capillary
Tube Lens Octapole Endcap
Mechanical
Pump
Skimmer
APCI Probe
Assembly
Turbo
Pump
Transfer Ions
Turbo
Pump
Ring
Electrode
Electron
Multiplier
+15 kV
Conversion
Dynode
Make Ions Desolvate Ions
Filter Ions
Detect
Ions

LCQ Probes used in Making Ions
◗ API-1 / ESI Probe (LCQ Classic)
◗ API-2 / ESI Probe (LCQ Deca / LCQ Duo)
◗ API-2 / Off Axis ESI Probe (Classic/Deca/Duo)
◗ APCI Probe (Same for API-1 and API-2)

ESI Ionization Process
ESI Ionization Process
Simplified Schematic

±5kV ±5kV
ESI Spray Cross Section
Nozzle
Gas Sheath
Needle
Liquid Sheath
Ion Plume

Aerosol Formation
Relative velocity between the sheath gas flow and the liquid flow
causes a shearing effect on the emanating large droplet, and
results in rapid droplet size reduction (spray)
Sheath Gas
Sample Tube
Field lines
Applied high voltage activates the
coulombic explosion process
Heated
Capillary

API-1 API 1 / ESI Probe Assembly
(LCQ Classic)

Cal Mix Tune Parameters
API-1 on LCQ Classic
◗ Infusion Flow Rate (µL/min.): 3-5
◗ Spray Voltage (kV): 4-6
◗ Spray Current (µA): 0.1-0.75
◗ Sheath Gas Flow Rate (arb): 30-60
◗ Aux. Gas Flow Rate (arb): 0
◗ Capillary Temperature: 200-250°C
◗ Probe Position : Pulled all the way back

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
ESI Source dialog and Status Panel

Troubleshooting the API1 Probe
◗ Spray Current too high: High Voltage (HV) shorting effects:
Leaks within ESI Probe
Problematic mixture of solvents/sample/etc.
◗ Erratic Spray Voltage: Shorted HV cable
Spay current at maximum
Bad HV supply
◗ Erratic spray: Sample tube
ESI needle
Sheath gas flow
◗ Contamination: Wipe spray shield around heated capillary
Wipe out ESI probe flange
Flush the ESI probe
Clean ESI probe interior parts
◗ Overall solution for 90% of source related Problems:
� good maintenance
(especially with the sample tube)
� use of divert valve

API-1 API 1 / ESI Probe Cross Section

Sample Tube Elongation
ESI Needle
Sheath Liquid
ESI Needle
ESI Needle
ESI Needle
ESI Needle
Polyimide
Polyimide
Sheath Liquid
Sheath Liquid
ESI Needle
ESI Needle
ESI Needle
Polyimide
Polyimide
Sheath Liquid
Fused Silica
Fused Silica
Fused Silica
Fused Silica
Sample
Sample
Elongation of polyimide
occurs when specific
solvents are adsorbed into
the sample tube.
The sample tube must be cut
to ensure good spray.
The sample tube must be cut
square to ensure good spray.
Best results can be achieved
by making the sample tube
flush with the ESI Needle.

Sample Tube Elongation Resolution
ESI Needle
Sheath Liquid
ESI Needle
Polyimide
ESI Needle
Polyimide
Sheath Liquid
ESI Needle
ESI Needle
Sheath Liquid
ESI Needle
Polyimide
Polyimide
Sheath Liquid
ESI Needle
Fused Silica
Fused Silica
Fused Silica
Fused Silica
Sample
Sample
The polyimide can be flamed
to avoid elongation; however,
make sure the end of the
fused silica is cut square.
Again, make sure the end of
the fused silica is cut square.
In this case, the fused silica
is cut on an angle. This will
produce poor spray.

Divert Valve Configuration
TRANSFER LINE FROM
DIVERT/INJECT VALVE
TRANSFER LINE
FROM LC PUMP
SYRINGE
PORT
WASTE
LINE
3
Front Panel Injections
L oa d
Detect or
4
2
5
DIVERT/
INJECT
VALVE
6
I nj ect
Waste
1
TRANSFER LINE
FITTING
SAMPLE
LOOP
A U X I L I A R Y
G A S
S H E A T H
G A S
S A M P L E
S H E A T H
L I Q U I D
H I G H
V O LT A G E
ESI SOURCE
GROUNDED
FITTING
HOLDER
SAMPLE TUBE

Divert Valve Configuration
TRANSFER LINE FROM
DIVERT/INJECT VALVE
TRANSFER LINE
FROM LC PUMP
FROM LC
PUMP
TO ION
SOURCE
Normal Applications
Load
Detector
3
WASTE
LINE
3
4
2
5
6
DIVERT/
INJECT
VALVE
4
2
5
TRANSFER LINE
FITTING
Inject
Waste
1
6
1
SHEATH
GAS
AUXILIARY SAMPLE
GAS
SHEATH
LIQUID
GROUNDED
FITTING
HOLDER SAMPLE TUBE
HIGH
VOLTAGE
ESI SOURCE
FROM LC
PUMP
TO ION
SOURCE
3
6
WASTE 4
WASTE
TO MS TO WASTE
2
5
1

Xcalibur Instrument Setup
Divert Valve Dialog

API-2 API 2 / ESI Probe Assembly
(LCQ Deca / LCQ Duo)

API-2 / ESI Probe Positions
4 3 2 1
Probe
Positions

LC Flow Rate
Infusion or LC at flow rates of

API-2 API 2 / ESI Probe
Exploded Pictorial

Cal Mix Tune Parameters
API-2 on LCQ Deca / LCQ Duo
◗ Infusion Flow Rate (µL/min.): 3-5
◗ Spray Voltage (kV): 4-6
◗ Spray Current (µA): 0.1-0.75
◗ Sheath Gas Flow Rate (arb): 20-40
◗ Aux. Gas Flow Rate (arb): 0
◗ Capillary Temperature: 200-250°C
◗ Probe Position : 2

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
(ESI Source dialog and Status Panel)

Troubleshooting the API2 Probe
◗ Contamination: Wipe spray shield around heated capillary
Wipe out ESI probe flange
Flush the ESI probe
◗ Erratic spray: Sample tube
Sheath gas flow
◗ Spray Current too high: Problematic mixture of solvents/sample/etc.
◗ Erratic Spray Voltage: Spray current at maximum
◗ Overall solution for 90% of source related Problems:
� Good maintenance
(especially with the sample tube)
� Use of divert valve

API-2 API 2 / ESI Probe Assembly
Cross Section

API-2 API 2 / Off Axis ESI Probe
(LCQ Deca / LCQ Duo / LCQ Classic)

API-2 API 2 / Off Axis ESI
Tangential Movement
Probe Geometry
ESI probe on a 25 degree slide
Ion flow
Heated
Capillary
matrix flow

API-2 API 2 / Off Axis ESI Guidelines
LC Flow Rates
Infusion or LC at flow
rates of 200 µL/min
Slide
Plate
Position
Operational Parameters
Probe
Position
(1 to 7)
Heated
Capillary
Temperature
Top 4 Typical setting:
250 °C
Top 4 Typical setting:
350 °C
Top 5 Typical setting:
350 °C
Sheath
Gas
Required
Typical setting:
10 to 30 units
Required
Typical setting:
80+ units
Required
Typical setting:
80+ units
Auxiliary
Gas
Not required
Typical
setting:
0 units
Required
Typical
setting:
10 to 20 units
Required
Typical
setting:
10 to 20 units

APCI Ionization Process

LCQ Classic APCI Probe

LCQ Deca / LCQ Duo APCI Probe

Typical APCI Tune Parameters
Conditions: Reserpine at 1ml/min
◗ Vaporizer Temp (°C): 400-550 (600 max.)
◗ Discharge Current (µA): 5 (10µA max.)
◗ Discharge Voltage (kV): 4-6kV (read back)
◗ Sheath Gas Flow Rate (arb): 50-80
◗ Aux. Gas Flow Rate (arb): 0-20
◗ Capillary Temp (°C): 125-250
◗ Capillary Voltage (V): 10-40
◗ Tube Lens Offset (V): 30-60
◗ Flow Rate capability: 50µL/min. - 2mL/min.

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
APCI Source dialog and Status Panel

Troubleshooting the APCI Probe
◗ Spray voltage erratic: Spray current should not exceed 20µA
Once 20µA level is reached, the spray
voltage will be lowered to compensate
for the 20µA threshold
◗ Spray current too high: Problematic mixture of solvents/sample/etc.
◗ Contamination: Bake out the probe for 10-60 minutes at 50ºC
above desired Vaporizer Temperature
◗ Lack of sensitivity: Make sure the corona needle is seated
and is not deformed

API-1 API 1 / API-2 API 2 APCI Probe
Cross Section

Ion Transfer / Desolvation Process
Overview
ESI Nozzle
(± ± 8 kV)
Solvent/Buffer
+
+
+
+
+
+
+
Heated Capillary
+ +
+
Ion
Stream

LCQ API Stack Gas Flow
1 Torr
×
×
Tube lens
Peek Holder
skimmer
10 -3 Torr

LCQ API Stack Assembly
Cross Section
Kalrez OO-Ring
OO-Ring
Ring
Capillary must be flush with the
Tube lens and Skimmer Mount.
Peek Bushing
Capillary Sleeve
Manifold
Manifold
Heated Capillary Specifications:
Heater Resistance: 14Ω
Platinum Sensor: 110Ω

Cal Mix Tune Parameters
LCQ API Stack
◗ Capillary Temp (°C)*: 200-250
◗ Capillary Voltage (V): 10-40
◗ Tube Lens Offset (V): 30-60
* Heated Capillary I.D.:
LCQ Classic / LCQ Duo: 400µm
LCQ Deca: 500µm

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
Vacuum dialog and Status Panel

Troubleshooting the API Stack
◗ Loss of Sensitivity: Clean skimmer and tube lens
◗ Low Convectron Gauge Pressure: Heated capillary plugged
◗ Cal Mix Contamination: Clean API probe and heated capillary area
◗ Spiky noise: Bent capillary tip or dirty heated capillary
◗ Constant Background: Possible heated capillary contamination
◗ Overall solution for 90% of API Stack related Problems:
� Good maintenance:
Caution:
Clean/Rinse the heated capillary region
and spray shield daily.
� Use of the divert valve
� Use of Spray Cap and Orthogonal
Sampling Adapter
Periodically empty the waste bottle to avoid potential back streaming of waste
solvent into the source region.

Orthogonal Spray Adapter
Configuration
ESI Probe
Buffer deposition
‘Orthogonal’ ion flow
Focusing ring Liquid drains
Heated capillary

Orthogonal Spray Adapter Guidelines
Operational Parameters
LC Flow Rate
Infusion or LC at flow
rates of

ESI Probe
Reduces Cal Mix contamination
Spray Cap
Configuration
Spray Cap
Capillary Sleeve
Heated capillary
Spray Shield
O-Ring Ring
Peek
Bushing
Spray Shield

Spiky Noise Characteristics
Single Noise Spike
S#: 4 RT: 0.27 AV: 1 T: + p Full ms NL: 2185325
Abundance
ve
elati
R
100
90
80
70
60
50
40
30
20
10
0
330.5
326.6 328.6 330.9
334.3
327.6 328.0 329.0
331.8
334.6
326 327 328 329 330 331 332 333 334 335
m/z
335.6

Spiky Noise Characteristics
S#: 4 RT: 0.27 AV: 1 T: + p Full ms NL: 7692800
100
90
80
70
60
Abundance 50
e
elativ
elativ
R 40
30
20
10
0
308.5
330.5
Particle Noise Spectrum of Cal mix
Spiky Noise at 10 uscans
524.4
536.3
195.2
262.7
413.3
553.7
690.9
812.2
959.6
872.7 1022.3
1122.1
1222.1
1322.1
1421.9
1522.0
1621.8
1721.9
200 400 600 800 1000
m/z
1200 1400 1600 1800
1821.8

Heated Capillary Cross Section
Bent Capillary Tip
Heated
Capillary
Bent Capillary Tip
+
+
+
+
+
+
+
+
+ +
+
+
+ +
+ +
+
+
+
Tube
Lens
Fixed
distance
Skimmer
With time, compound will
neutralize out on the skimmer.
This will spot will eventually
need to be cleaned; otherwise,
field affects can reduce
sensitivity.
Avoid bending the tip of the heated
capillary. The tip of the capillary
must remain off axis to the
skimmer; otherwise, spiky noise or
reduced sensitivity can occur.
The distance between the end of
the capillary and the skimmer
opening must remain fixed.

Ion Transfer Overview
Deca Ion Optic System
Ions will be trapped
in stable trajectories
Ion Stream
from skimmer
Transfer
Array
Fundamental RF
on Ring
Ion Trap

Octapole 1 Offset: LCQ / LCQ Duo
Quadrupole 1 Offset: LCQ Deca
Ion Optics
Gating Ions into the Ion Trap
Multipole RF
Octapole 2 2 Offset:
Offset:
LCQ / LCQ Duo / LCQ Deca

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
Ion Optics dialog and Status Panel

Cal Mix Tune Parameters
LCQ Ion Optics
Octapole 1 Offset (V) for Classic/Duo: -1 to -5
Octapole 1 Offset (V) for Deca: -4 to -9
Lens Voltage (v): -16 to -50
Octapole 2 Offset (V) for Classic / Duo: -5.5 to -10
Octapole 2 Offset (V) for Deca: -7 to -15
Octapole RF Amplitude (V p-p): 400
Entrance Lens (V) for the Deca only: -35 to -60 V
Troubleshooting:
Loss of Sensitivity: * Clean octapoles(multipoles) and lens
Octapole Diagnostic errors: * Tune multipole RF prior to running diagnostics.
* Multipole RF tune now performed in calibration
for all LCQs run with Xcalibur.

760 torr
LCQ Classic Optics
Typical Operating Pressures
1.0 torr 1.7x10 -3 torr
30 m 3 /hr
100 L/sec
2.0 x10 -5 torr (1.0x10 -5 torr He)
3.5x10 -3 torr He
220 L/sec

LCQ Duo Optics
Typical Operating Pressures/Comparison to LCQ Classic
1.0 torr 1.7x10-3 760 torr torr 2.0 x10-5 torr (1.0x10-5 torr He)
30 m 3 /hr
100 L/sec
3.5x10 -3 torr He
220 L/sec

LCQ Deca Optics
Typical Operating Pressures/Comparison to LCQ Classic
1.3 torr 1.7x10-3 760 torr torr 2.0 x10-5 torr (1.0x10-5 torr He)
60 m 3 /hr
100 L/sec
3.5x10 -3 torr He
220 L/sec

Potential
Potential Energy Diagram
LCQ and LCQ Duo
0
4000
20
Source CID
= 20%
50
0
-3
-23
-20
-40
-7
-27
-10
-30
-15000

Potential
Potential Energy Diagram
LCQ Deca
0
4000
50
20
0
-5
-20
-7 -10
-50
-15000

RF Tune Diagnostic Dialog
RF Tune Diagnostic Dialog
LCQ Deca

Tune Multipole RF
Tune Multipole RF
LCQ Deca

Multipole RF Tune
in Calibration Process
Multipole RF Tune
verification performed
prior to calibration.

LCQ Deca Ion Optics
Specifics Features
Square Square Quadrupole
Quadrupole
New New Endcap Endcap Electrodes
Electrodes
New New Entrance Entrance Lens
Lens
New New Inter-Octapole Inter-Octapole Inter Octapole Lens
Lens

Split Multipole
Deca / Duo Configurations
Split Square Quadrupole
LCQ Deca
Deflection - set to +132
Transmit - both set to Offset Value
Split Octopole
LCQ Duo
Deflection - set to -132

Relative Abundance
Relative Abundance
100
90
80
70
60
50
40
30
20
10
100
90
80
70
60
50
40
30
20
10
0
0
Effect of Split Multipole
No Split
Multipole
200 300 400 500 600 700 800 900
m/z
With Split
Multipole
MRFA
m/z 524
(with isotopes)
MRFA
m/z 524
(with isotopes)
200 300 400 500 600 700 800 900

-DC
LCQ Deca Noise Reduction
Split DC on Scan out
No Split
+DC
DECA
LCQ
Pulsed lens

Overview of Ion Separation
Quadrupole Ion Trap
Ion Stream
from Transfer Array
Ion Trap
Fundamental (Ring Electrode) and Resonance Ejection (End Caps) RF potential
are ramped to sequentially eject ions from the Ion Trap

Mass Selective Instability
Simplified Overview
0.2
0.1
0
-0.1
-0.2
-0.3
-0.4
-0.5
-0.6
-0.7
a z
0.2
β z
0.3
0.4 0.5 0.6 0.7 0.81.0
0
0.1
0.2
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6
0.3
0.4
0.5
β
0.6 r
0.7
0.8
0.9
1.0
q z-edge = 0.908
q z = k v
(m/z)
q z

Ion Trap Mass Analyzer
Ion Trap Mass Analyzer
Filter Ions

Quadrupole Ion Trap
Operational Parameters
◗ Main RF: 16.2 kV p-p max.
(760kHz)
◗ Resonance Ejection RF: 80 V p-p max.
(frequency Varies)
◗ Waveform RF: 160 V p-p max.
(Arbitrary)
◗ Trap Offset: 10 V fixed
◗ Exit Lens: at Ground potential
◗ Helium gas consumption: 1 cc/min. under
vacuum

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
Injection Control dialog and Status Panel

Troubleshooting the Ion Trap
◗ Loss of Sensitivity: Clean End caps and ring electrode
◗ High Mass Noise: Clean spacer rings and endcaps.
◗ Lack of sensitivity/resolution/mass stability:
Lack of helium or Air leak
Notes:
Full scan target: -Set to 5X10e7 for best sensitivity
in the positive ion mode.
-For best mass stability results, set to 2X10e7.
-Set (2-3) times less in the negative ion mode.
A typical value of 1X10e7 should be used.
Positive/Negative switching: -Xcalibur allows for separate tune files.
Pos/Neg scan segments can be used.
ZoomScan target: -Set to (1-3)X10e6 for compounds with
multiple charge.
-Singly charged compounds will exhibit a
slightly higher target.
-For calibration with Cal Mix, set to 1X10e7.

Tune RF Frequency
LCQ Deca
Measure RF
Frequency
Detected RF
RF Frequency
Well
Standing Wave
Ratio
Switch Status

Tune RF Modulation
LCQ Deca
Standing
Wave
Ratio
Switch Status
Detected RF
Highest Mass
RF Modulation
Upper
and
Lower
Ranges
RF Modulation

S#: 34 RT: 1.11 AV: 1 T: + p ms NL: 10610
Abundance
ve
elati
R
100
90
80
70
60
50
40
30
20
10
0
High Mass Noise
901.9
951.5
1196.3
1229.1
1336.0
1504.0
200 400 600 800 1000 1200 1400 1600
m/z
1641.4
1628.6
High Mass noise due to a RF electrical discharge from inside the manifold.
17

Affects of Helium on Spectra
Helium Helium flowing flowing into into trap
trap
S#:1 RT:0.00 AV:1 SM:7G NL:2.50E7
T: + p Full ms
Relative Abundance
100
80
60
40
20
0
524.3
525.3
514 516 518 520 522
m/z
524 526 528
Helium Helium shut shut off off and and not not flowing flowing into into trap
trap
S#:1 RT:0.02 AV:1 SM:7G NL:9.70E6
T: + p Full ms
Relative Abundance
100
80
60
40
20
0
520.7
521.8
521.2
522.6 523.0 523.0
523.9
514 516 518 520 522
m/z
524 526 528
S#:23-32 RT:0.71-1.00 AV:10 SM:7G NL:5.61E7
T: + p Full ms
Relative Abundance
100
80
60
40
20
0
195.15
524.26
1322.06
1222.14
1522.04
1621.97
1721.89
1821.95
1122.21 1921.88
1022.09
500 1000
m/z
1500 2000
S#:23-32 RT:0.39-0.54 AV:10 SM:7G NL:2.80E7
T: + p Full ms
Relative Abundance
100
80
60
40
20
0
192.17
1320.95
1620.79
1520.26
1720.44
1220.75
523.01 1919.96
1120.90
500 1000
m/z
1500 2000

Ion Detection

2-Particles
enter the
multiplier
Electron Multiplier
Detection System
Cathode
Applied High
Voltage
Anode cup
~
Two particles formed when an ion
ejected from the Ion Trap hits the
dynode. Dynode particles enter the
multiplier.
Each particle hits the surface of the
multiplier resulting in the ejection of
two more particles.
The cascading effect of this process
will produce a charge on the anode
cup.
This charge represents the signal
produced by the ion.
Signal to Data system.

LCQ Deca Tune Plus Window
LCQ Deca Tune Plus Window
Ion Detection System dialog and Status Panel

Detection System Parameters
◗ Dynode Voltage: 15kV (fixed)
◗ Electron Multiplier: 2500 Volts max.
Note:
– Multiplier voltage is a calibrated parameter.
– For best results, the multiplier should not be set manually.
Troubleshooting:
No peaks on Classic: Check multiplier voltage
Switch dynode polarity
Check for ions
No peaks on Deca/Duo: Check Multiplier and Dynode Voltages.
Noisy spectra with heated capillary capped off:
Potential Dynode noise
Clean Dynode Cup

Dynode Noise
◗ Dynode noise occurs over time.
◗ A noisy baseline with the heated
capillary capped off is the
symptom.
◗ This is due to an accumulation of
material that can build up in the
dynode cup over time.
◗ The cup should be cleaned when
this occurs.

Voltage
Life Time of the
Electron Multiplier
Time
The voltage needed to produce gain on the multiplier should show a linear increase with time.

LCQ Recommended Maintenance Schedule
Daily:
Morning Recommendations:
◗ Check the convectron and ion gauge pressures. Make sure the
vacuum system is operational.
◗ Check the fused silica sample tube. Make sure the fused silica has
not elongated.
◗ Remove the septum cap.
◗ Check the convectron and ion gauges pressure again. Make sure
the vacuum pressures are still OK.
◗ Start your analysis.

LCQ Recommended Maintenance Schedule
Daily:
Evening Recommendations:
◗ Put the system in the stand-by mode.
◗ Turn off the Ion Gauge and rinse the Heated Capillary with methanol
or an appropriate solvent. When finished, turn Ion Gauge back on.
◗ Cap off the heated capillary with the septum cap.
◗ Secure the API probe to the API stack.
◗ Make sure the solvent waste bottle is empty.
◗ Open the mechanical pump ballast for roughly 0.5 hour.
◗ While the pump is ballasting, maintain (move, delete, and/or copy)
files at the LCQ computer.
◗ If bottled nitrogen gas is being used, check the nitrogen gas.
◗ After 0.5 hour, close the ballast valve.

LCQ Recommended Maintenance Schedule
Weekly:
For normal operation:
◗ Check the mech. pump oil level.
◗ Fill the mech. pump as needed.
◗ If through put is high (running 24 hours a day), schedule
one day a week to:
- Change the mech. pump oil.
- Clean the skimmer and tube lens.

LCQ Recommended Maintenance Schedule
Monthly:
Every Month:
◗ Check the helium gas tank pressure. Replace as needed.
◗ Check the nitrogen gas tank (Dewer) pressure. Replace as needed.
◗ Nitrogen gas consumption:
– Typical: 3-6 L/min
– Worse case scenario associated with choice of Nitrogen Generator:
15L/min (28SCFH at 100psi and 99% purity) for LCQ Classic
30L/min (56SCFH at 100psi and 99% purity) for Duo / Deca
◗ Check the LCQ calibration.
◗ Check the air filter. Clean if necessary.
◗ Possibly, change the mech. pump oil.

LCQ Recommended Maintenance Schedule
Every 3 months:
Change the mech. pump oil.
6 months to a year:
Change the turbo pump oil.
Notes
- The API stack and analyzer should be clean as needed.
- If the sensitivity starts to drop off and can not be
restored, clean the API stack (and analyzer) as needed.

Overview of Diagnostics
◗ Tests and Tools provided:
– Static Tests
– Dynamic Tests
– Diagnostic Tools
– Research Tools
◗ Limitations:
– Provides status of electronics components
– Does not provide readbacks from the actual source element
Can not detect a broken or loose connection.
◗ Prior to running the dynamic diagnostics:
– Tune all RF components (Octapole/Multipole and Main RF).
– Make sure the system in the ON mode.
– Make sure the API Probe is bolted to the API stack. This allows the
high voltage to be activated.

Run All Diagnostics
Run All Diagnostics
Dynamic and Static Tests

Static Results from Status Test
+15 V - Pass
+150 V - Pass
+205 V - Pass
+24 V - Pass
+28 V - Pass
+35 V - Pass
+36 V - Pass
+5 V - Pass
-15 V - Pass
-150 V - Pass
-205 V - Pass
-28 V - Pass
8 kV PS voltage - Pass
Ambient temp. - Pass
Analyzer temp. - Pass
Aux gas flow - Pass
Capillary temp. - Pass
Capillary voltage - Pass
Convectron - Pass
Detected RF - Pass
Dynode voltage - Pass
Entrance lens - Pass
Intermultipole lens - Pass
Ion gauge - Pass
Main RF DAC - Pass
Multiplier setting - Pass
Multiplier voltage - Pass
Multipole 1 offset - Pass
Multipole 2 offset - Pass
Multipole RF mod. - Pass
Multipole det. RF - Pass
Multipole RF amp. out - Pass
RF amp. output - Pass
RF det. temp. - Pass
RF gen. temp. - Pass
RF modulation - Pass
Sheath gas flow - Pass
Trap DC Offset - Pass
Tube/gate lens - Pass

Dynamic Results
RF Test
Starting All Diagnostics Scan Tests
16:18:30: Start scan readback test on device Auxiliary amplitude (V) -- 0 to 83.2
16:18:35: Scan readback test ended
16:18:37: Result: PASSED
16:18:37: Start scan readback test on device Main RF DAC (16-bit) -- 0 to 65535
16:18:42: Scan readback test ended
16:18:44: Result: PASSED
16:18:44: Start scan readback test on device Vernier det. RF amp. (V) -- 0 to 65535
16:18:48: Scan readback test ended
16:18:50: Result: PASSED
16:18:50: Start scan readback test on device Vernier RF DAC (16-bit) -- 0 to 65535
16:18:55: Scan readback test ended
16:18:57: Result: PASSED
16:18:57: Start scan readback test on device Multipole RF DAC (V) -- 0 to 1000
16:19:02: Scan readback test ended
16:19:04: Result: PASSED

Dynamic Test
Dynamic Test
Graphical Output

Starting All Diagnostics Scan Tests
Dynamic Results
Lenses Test
16:19:04: Start scan readback test on device Multipole 1 offset (V) -- -132 to 132
16:19:09: Scan readback test ended
16:19:11: Result: PASSED
16:19:11: Start scan readback test on device Multipole 2 offset (V) -- -132 to 132
16:19:16: Scan readback test ended
16:19:17: Result: PASSED
16:19:17: Start scan readback test on device Multipole lens (V) -- -132 to 132
16:19:22: Scan readback test ended
16:19:24: Result: PASSED
16:19:24: Start scan readback test on device Multipole det. RF amp. (Vp-p) -- 0 to 1000
16:19:29: Scan readback test ended
16:19:31: Result: PASSED

Dynamic Results
Ion Detection Test
Starting All Diagnostics Scan Tests
16:19:31: Start scan readback test on device Trap Offset (V) -- -132 to 132
16:19:36: Scan readback test ended
16:19:38: Result: PASSED
16:19:38: Start scan readback test on device Tube gate (V) -- -200 to 198.01
16:19:43: Scan readback test ended
16:19:45: Result: PASSED
16:19:45: Start scan readback test on device Multiplier (V) -- 0 to -2200
16:19:58: Scan readback test ended
16:20:00: Result: PASSED

Dynamic Results
API Source Test
Starting All Diagnostics Scan Tests
16:20:00: Start scan readback test on device Auxiliary gas flow (arb) -- 0 to 60
16:21:35: Scan readback test ended
16:21:37: Result: PASSED
16:21:37: Start scan readback test on device Sheath gas flow (arb) -- 20 to 100
16:23:20: Scan readback test ended
16:23:22: Result: PASSED
16:23:22: Start scan readback test on device Capillary Voltage (V) -- -132 to 132
16:23:27: Scan readback test ended
16:23:28: Result: PASSED
16:23:28: Final result: PASSED

Power Supplies
Power Supplies
Static Diagnostic

API and Temperature
API and Temperature
Static Diagnostic

Lenses
Static Diagnostic

RF
RF-1
Static Diagnostic

RF
RF-2
Static Diagnostic

Calibration
Diagnostic Tool

Toggles/Detector
Diagnostic Tool

Instrument Settings
Instrument Settings
Diagnostic Tool

Graphs
Plotting Conversion Dynode Voltage

Graphs
Plot Tube Lens Calibration

Triggers
Research Tool

Front Panel LEDs
Classic/Deca

Front Panel LEDs
Front Panel LEDs
Definition of Classic/Deca
�Power: Indication of the digital power
– Should be green unless the power to the LCQ is off or there has been a failure.
�Vacuum: Indicates that the vacuum is OK.
– (Convectron gauge, ion gauge, and external switch are all in the correct state.)
– Should be green. If any one of the inter-locks is not logically correct, the LED will be off.
�Communication: Indicates communication between the on-board AT CPU and the NT
computer.
– Will be green if the two computers are communicating.
– Will be yellow if the on-board AT is active but not communicating with the NT computer.
– Will be off if the LCQ is off or if a failure has occurred.
�System: Indicates the status of the LCQ.
– Will be green if the LCQ is in the On mode. High voltage is applied.
– Will be yellow if the LCQ is in the standby mode. High voltages are off.
– Will be off if the LCQ is in the Off mode. Most of the power supplies are off.
�Scan: Indicates that the LCQ is in the On mode and Scanning.
– Will be blue and flashing when the LCQ is collecting data.
– Will be off if the LCQ is either in the On or Standby mode. Also will be off if the LCQ is
off.

Front Panel LEDs
LCQ Duo

LCQ Duo Front Panel LEDs
Definition
�Power: Indication of the digital power
– Should be green unless the power to the LCQ is off or there has been a failure.
– Will flash yellow indicating a Warning condition for the on-board CPU temperature .
– Will be solid yellow indicating a Fatal condition. The MS will be held in a Reset mode until
the temperature problem has been resolved.
�Vacuum: Indicates that the vacuum is OK.
– (Convectron gauge, ion gauge, and external switch are all in the correct state.)
– Should be green. If any one of the inter-locks is not logically correct, the LED will be off.
�Communication: Indicates communication between the LCQ AT
CPU and the NT computer.
– Will be green if the two computers are communicating.
– Will be yellow if the on-board AT is active but not communicating with the NT computer.
– Will be off if the LCQ is off or if a failure has occurred.

LCQ Duo Front Panel LEDs
Definition
� System: Indicates the status of the LCQ.
− Will be green if the LCQ is in the On mode. High voltage is applied.
− Will be yellow if the LCQ is in the standby mode. High voltages are off.
− Will be off if the LCQ is in the Off mode. Most of the power supplies are off.
� Scan: Indicates that the LCQ is in the On mode and Scanning.
− Will be blue and flashing when the LCQ is collecting data.
− Will be off if the LCQ is either in the On or Standby mode. Also will be off
if the LCQ is off.
� Syringe Pump: Indicates the status of the syringe pump.
− Will be green if the syringe pump on.
− Will be yellow when the pump has reached it end of travel.