Advances in Food Mycology

Evaluation of Molecular Methods for the Analysis of Yeasts in Foods 79
Species-specific primers
Universal and species-specific Pathogenic yeasts C. neoformans, Fell (1995)
primers (V3 region of LSU) T. cutaneum, R. mucilaginosa
Universal and species-specific primers Detection of several Candida species Mannarelli and Kurtzman (1998)
(D1/D2 region of LSU)
1) species-specific primer pairs Identification of Z. bailii, Z. bisporus, Sancho et al. (2000)
Z. rouxii and T. delbrueckii isolates from fruit
2) one species-specific, the other
universal (ITS region)
Nested PCR D. bruxellensis strains from isolates and sherry Ibeas et al. (1996)
Multiplex PCR 5 primers; 1 universal, Identification of Dekkera isolates; Egli and Henick-Kling (2001)
4 species-specific (ITS region) differentiation of B. bruxellensis,
B. anomala, B. custersianus, B. naardenensis
PCR and RT-PCR Detection of B. bruxellensis/B. anomalus Cocolin et al. (2004)
Specific primers (D1/D2 26S LSU) from wine samples
RT-PCR; Specific primer pairs (cs 1) gene Detection of viable C. krusei in fruit juice Casey and Dobson (2003)
RT-PCR; Specific primers (ITS Identification of S. cerevisiae and Josepa et al. (2000)
and LSU region) S. bayanus/pastorianus isolates
PCR, Multiplex PCR; 4 primers; Detection of S. cerevisiae, S. bayanus Torriani et al. (2004)
2 species-specific pairs (YBR033w region) and S. pastorianus
Real time PCR
Primer pairs (D1/D2 LSU) Detection and enumeration of Phister and Mills (2003)
D. bruxellensis in wines
Primer pairs (rad4 gene) Detection and quantification of Delaherche et al. (2004)
B. bruxellensis in wines
Specific primer pairs (cs 1) gene Quantification of C. krusei from fruit juice Casey and Dobson (2004)
Universal primer pairs ITS3 and ITS4 Differentiation of Z. bailii, Z. rouxii, C. krusei, Casey and Dobson (2004)
(5.8S and ITS2) R. glutinis and S. cerevisiae by difference in Tm