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Advances in Food Mycology

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Evaluation of Molecular Methods for the Analysis of Yeasts <strong>in</strong> <strong>Food</strong>s 73<br />

Table 1. Application of gene sequenc<strong>in</strong>g technology to the identification of species<br />

of food and beverage yeasts<br />

Region Application References<br />

18S Phylogenetic relationships;<br />

Zygosaccharomyces and<br />

Torulaspora species James et al. (1994)<br />

Brettanomyces, Dekkera, Cai et al. (1996)<br />

Debaryomyces, Kluyveromyces<br />

species<br />

Candida, Pichia, Citeromyces species Suzuki and Nakase (1999)<br />

Saccharomyces genus; new species James et al. (1997)<br />

S. kunashirensis, S. mart<strong>in</strong>iae<br />

Saccharomyces sensu lato group; Mikata et al. (2001)<br />

new species S. naganishii,<br />

S. humaticus, S. yukushimaensis<br />

18S, ITS Phylogenetic relationships of Naumov et al. (2000)<br />

Saccharomyces sensu stricto<br />

complex; new species<br />

S. cariocanus, S. kudriavzevii,<br />

S. mikatae<br />

18S; 834- Identification of yeast from Cappa and Cocconcelli (2001)<br />

1415 dairy products<br />

D1/D2 Systematics of ascomycetous yeasts Kurtzman and Robnett (1998)<br />

of 26S Systematics of basdidiomycetous Fell et al. (2000)<br />

yeasts<br />

D1/D2 of Systematics of basdidiomycetous Scorzetti et al. (2002)<br />

26S, ITS yeasts<br />

D1/D2 of Candida davenportii sp. nov. from Stratford et al. (2002)<br />

26S a wasp <strong>in</strong> a soft-dr<strong>in</strong>k production<br />

facility<br />

D1/D2 of Tetrapisispora fleetii sp. nov. from Kurtzman et al. (2004)<br />

26S, ITS a food process<strong>in</strong>g plant<br />

D1/D2 Identification of yeast species;<br />

of 26S <strong>in</strong> Sicilian sourdough Pulvirenti et al. (2001)<br />

<strong>in</strong> orange juice Arias et al. (2002)<br />

<strong>in</strong> spontaneous w<strong>in</strong>e fermentation van Keulen et al. (2003)<br />

from bark of cork oak Villa-Carvajal et al. (2004)<br />

from Malbec grape berries Comb<strong>in</strong>a et al. (2005)<br />

from fermentation of West Jespersen et al. (2005)<br />

African cocoa beans<br />

contam<strong>in</strong>ant <strong>in</strong> carbonated orange P<strong>in</strong>a et al. (2005)<br />

juice production cha<strong>in</strong><br />

ITS Phylogenetic relationships of Belloch et al. (2002)<br />

Kluyveromyces marxianus group<br />

ITS Phylogenetic relationships of James et al. (1996)<br />

Zygosaccharomyces and<br />

Torulaspora species

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