Advances in Food Mycology

APPENDIX 1 – MEDIA
All media are sterilized by autoclaving at 121°C for 15 min unless
otherwise specified. Czapek agars may be made from basic ingredients,
or their production can be simplified by the use of Czapek concentrate
and Czapek trace metal solution which contain the required
additional salts in the specified concentrations. Both options are provided
in this appendix.
AFPA: Aspergillus flavus and parasiticus agar (Pitt et al., 1983; Pitt
and Hocking 1997)
Peptone 10 g
Yeast extract 20 g
Ferric ammonium citrate 0.5 g
Chloramphenicol 100 mg
Agar 15 g
Dichloran 2 mg
(0.2% w/v in ethanol, 1 ml)
Water, distilled 1 litre
After addition of all ingredients, sterilise by autoclaving at 121°C
for 15 min. Final pH 6.0-6.5.
CMA: Corn Meal agar (Samson et al., 2004).
Add 60 g freshly ground cornmeal to 1 litre water. Heat to boiling
and simmer gently for 1 h. Strain through cloth. Make volume up to
1 litre with water, add 15 g agar, heat to dissolve agar, then autoclave
for 15 min at 121°C. Also available commercially.
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