LabAutomation 2006 - SLAS

LabAutomation2006
12:00 pm Tuesday, January 24, 2006 Track 5: Frontiers Beyond BioPharma Room: Sierra/Ventura
Wyndham Palm Springs Hotel
Gary Gust
Co-Author(s)
Osmetech Molecular Diagnostics
M. Reed
Pasadena, California
A. M. Aguinaldo
gary.gust@osmetech.com
L. Cheung
Y. Liu
J. Bumbarger
D. Wurtz
W.A. Coty
Cystic Fibrosis Carrier Screening Test Performed Using a Microarray Platform Based
on Electrochemical Detection of DNA Hybridization
The eSensor ® DNA detection technology enables genotyping of multiple genetic disease markers using electrochemical detection of
nucleic acid hybridization. Each eSensor cartridge contains an array of gold electrodes, each containing a sequence-specific capture
probe covalently linked to the gold surface. Each target nucleic acid is bound to its appropriate electrode through sequence-specific
hybridization to its capture probe, and then detected by hybridization of ferrocene-labeled signal probes and interrogation by alternating
current voltammetry. Each gold electrode is coated with an insulating monolayer which minimizes non-specific binding and prevents
signaling of unbound signal probes, eliminating the need for a wash step prior to detection. A cystic fibrosis (CF) carrier detection test
has been developed for the eSensor system, providing multiplex amplification and detection of carriers for the 23 mutations of the CF
transmembrane regulator gene recommended by the American College of Medical Genetics. Studies performed at external sites with 486
samples demonstrated an overall concordance of individual mutation calls with DNA sequencing of 99.0%, with a miscall rate of 0.02%.
3:00 pm Tuesday, January 24, 2006 Track 5: Frontiers Beyond BioPharma Room: Sierra/Ventura
Wyndham Palm Springs Hotel
Ryan Rodgers
Co-Author(s)
National High Magnetic Field Lab
Geoffrey C. Klein, Florida State University
Tallahassee, Florida
Tanner M. Schaub, National High Magnetic Field Lab
rodgers@magnet.fsu.edu
Donald F. Smith, Florida State University
Petroleomics: Mass Spectrometry Returns to Its Roots
Sunghwan Kim, National High Magnetic Field Lab
Jeremiah M. Purcell, Florida State University
Christopher L. Hendrickson, National High Magnetic Field Lab
Alan G. Marshall, Florida State University
Ultrahigh-resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) has recently spawned the new field
of “Petroleomics”, specifically defined as the relationship between the chemical composition of petroleum derived material and its
physical properties. The inherent high mass accuracy and high mass resolving power of FT-ICR MS provides the elemental composition
assignment of thousands of different polar heteroatom containing species from a single crude oil. Until very recently, the FT-ICR analysis
of complex, petroleum derived materials has been limited to polar heteroatom containing species amenable to ElectroSpray Ionization
(ESI). Here we present the latest developments in FT-ICR mass spectral analysis of crude oils and asphaltenes along with the latest
instrument developments that expand the analysis to include nonpolar, aromatic species found in crude oil. Included are initial results from
a commercial Atmospheric Pressure PhotoIonization (APPI) source (nonpolar aromatic species) and recent results from ESI FT-ICR MS
analysis of “heavy ends” (polar aromatic species). For a single oil sample, positive and negative mode ESI, as well as positive mode APPI
FT-ICR results are presented. Automation of the data acquisition procedure is also discussed. All heteroatom containing (ESI and APPI) and
PAH (APPI) classes are identified and their corresponding type and carbon number distributions presented. The level of detail provided by
FT-ICR MS analysis is unrivaled and amazingly, complete analysis (polars, nonpolars, aromatics and semi-volatile compounds) consumes
less than a drop of oil.
Work supported by NSF CHE-99-09502, Florida State University, and the National High Magnetic Field Laboratory in Tallahassee, FL
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