LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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<strong>LabAutomation</strong><strong>2006</strong><br />
12:00 pm Monday, January 23, <strong>2006</strong> Track 5: Frontiers Beyond BioPharma Room: Sierra/Ventura<br />
Wyndham Palm Springs Hotel<br />
Jeffrey Hurst<br />
Hershey Foods Laboratory<br />
Mt. Gretna, Pennsylvania<br />
gretnasci@aol.com<br />
Challenges to Laboratory Automation in Consumer Product Industries<br />
The early implementation of lab robotics as an evolutionary event in lab automation was meet with a great deal of excitement and interest<br />
in all industry segments. Over the years, as in all new technologies the excitement and interest waned especially those industry segments<br />
including the Consumer Product Industries who had initially been leaders. Presently, automation is essentially limited to phrama and related<br />
industry segments. This presentation will provide some historical perspective on this phenomena, discuss some potential reasons for this<br />
decline and provide some opportunities for lab automation in this industry group<br />
3:00 pm Monday, January 23, <strong>2006</strong> Track 5: Frontiers Beyond BioPharma Room: Sierra/Ventura<br />
Wyndham Palm Springs Hotel<br />
Amy Herr<br />
Co-Author<br />
Sandia National Labs<br />
Victor C. Rucker<br />
Livermore, California<br />
aeherr@sandia.gov<br />
Sandia National Labs<br />
Antibody Microarrays for Native Toxin Detection<br />
Timely detection of toxins in biological samples is important in assessing bio-security concerns. As a means to address such concerns,<br />
we have developed antibody-based microarray techniques for the multiplexed detection of cholera toxin (CT), diphtheria toxin (DT), anthrax<br />
lethal factor (LF) and protective antigen (PA), Staphylococcus aureus enterotoxin B (SEB), and tetanus toxin (TTC) in spiked samples. While<br />
the assay method makes use of DNA microarraying equipment and techniques, the approach enables direct protein quantitation.<br />
Two detection schemes were investigated: (1) a direct assay in which fluorescently labeled toxins were directly captured by the antibody<br />
array and (2) a competition assay using unlabeled toxins as reporters for the quantification of native toxins. In the direct assay, fluorescence<br />
measured at each array element is correlated with labeled toxin concentration to yield baseline binding information (Langmuir isotherms,<br />
affinity constants). The competition assay yields information on the presence, identity, and concentration of toxins, without the need<br />
for fluorescently labeling toxins in a sample. Calibration curves and detection limits were established for both assay formats. While the<br />
sensitivity of the direct assay is superior to the competition assay, detection limits for unmodified toxins in the competition assay are<br />
comparable to values reported for sandwich-format immunoassays.<br />
To highlight the potential of the competition assay for native toxin detection in biological samples, we conclude with a straightforward,<br />
multiplexed assay for the differentiation and identification of native S. aureus enterotoxin B and tetanus toxin in spiked, dilute serum<br />
samples.<br />
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