LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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Where Laboratory Technologies Emerge and Merge<br />
9:30 am Wednesday, January 25, <strong>2006</strong> Track 3: High-Throughput Technologies Room: Learning Center<br />
Wyndham Palm Springs Hotel<br />
James Myslik<br />
Bristol-Myers Squibb Co.<br />
Wallingford, Connecticutt<br />
james.myslik@bms.com<br />
Automation of Bioassays at BMS: Strategy and Implementation<br />
For the past five years, BMS Applied Biotechnology has been pursuing a HTS technology strategy that emphasizes flexibility, continuous<br />
flow and enablement of scientists to develop, operate and troubleshoot their assays independently. To achieve this, we have been building<br />
a platform that integrates a collection of commercially available and BMS-built sample handling, data collection and data processing<br />
modules into a user-defined workflow. Processed data is delivered in real time to the assay scientist for review and analysis. In striving for<br />
continuous flow, have learned lessons that have enabled us to leverage our HTS technologies for use in the central lead optimization cycle<br />
of drug discovery.<br />
10:00 am Wednesday, January 25, <strong>2006</strong> Track 3: High-Throughput Technologies Room: Learning Center<br />
Wyndham Palm Springs Hotel<br />
Tom Onofrey<br />
Nanostream, Inc.<br />
Pasadena, California<br />
nilma.rubin@nanostream.com<br />
Micro Parallel Liquid Chromatography (µPLC) for Confirmation Screening and<br />
Secondary Assays<br />
Researchers seek tools to confirm hits and eliminate false positives from screening studies. In addition, assay development can be both<br />
costly and time-consuming. This talk presents data for separation-based assays from a micro parallel liquid chromatography (µPLC)<br />
system, a high-throughput variant of HPLC. The system—which includes an autosampler, UV absorbance and fluorescence detectors,<br />
software, and 24-column microfluidic cartridges—facilitates assay development, offers quantitative information about hits, and provides<br />
additional information on compound purity and solubility. This talk will present data from assays used in lead identification and for phase 2<br />
metabolism studies to demonstrate the advantages of reduced interference and a ratio-metric readout. The system will be compared to<br />
plate-reader approaches as well as to other analytical solutions.<br />
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