LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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<strong>LabAutomation</strong><strong>2006</strong><br />
12:00 pm Tuesday, January 24, <strong>2006</strong> Track 3: High-Throughput Technologies Room: Learning Center<br />
Wyndham Palm Springs Hotel<br />
Sophia Liang<br />
Co-Author(s)<br />
Aurora Biomed<br />
Sikander Gill<br />
Vancouver, Canada<br />
Rajwant Gill<br />
sophia@aurorabiomed.com<br />
David Wicks<br />
Joy Goswami<br />
Dong Liang<br />
Performance Evaluation of a Robotic Workstation for HTS Flux Assays<br />
The development and validation of a robotic system was carried with the objective of improving efficiency and increasing the level of<br />
automation and miniaturization of assays. The workstation was employed to carry “walk away” cell based assay using cells expressing an<br />
ion channel. The data was compared with manually performed assay for various parameters like IC50 of a panel of blockers of the ion flux,<br />
SEM to determine the variability among replicates, and Z’ values to evaluate the overall quality of the assay. The data would be discussed<br />
in view of the demands for robotics in drug discovery.<br />
3:00 pm Tuesday, January 24, <strong>2006</strong> Track 3: High-Throughput Technologies<br />
Co-Author(s)<br />
Room: Learning Center<br />
Wyndham Palm Springs Hotel<br />
Jay Strum<br />
Iain Uings<br />
GlaxoSmithKline<br />
James Ward<br />
Research Triangle Park, North Carolina<br />
Paula Selley<br />
jay.c.strum@gsk.com<br />
Jason Holt<br />
Jodi Goodwin<br />
Frank Maurio<br />
Rusty Czerwinski<br />
Lazar Arulnayagam<br />
GlaxoSmithKline<br />
Automation and Miniaturization of TaqMan Assays to Support Drug Discovery<br />
There are many potential applications for quantitative real time PCR in the drug discovery process. This gene expression platform offers<br />
several advantages such as sensitivity, a large dynamic range of quantitation and flexibility. To minimize cost and increase throughput, we<br />
have worked to automate each step of our workflow and simultaneously miniaturize TaqMan assays. We have successfully implemented an<br />
automated storage and retrieval system for primers and probes supported by a LIMS system, automated total RNA isolation and reduced<br />
our reaction volumes to 2 ul using a combination of liquid handling robots. Finally, we have automated data exporting and built a data<br />
analysis tool that allows the rapid analysis, presentation and storage of data in a centralized database accessible to all GSK scientists. In<br />
this presentation, we will discuss our processes and show the successful application of the system to several drug discovery efforts.<br />
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