LabAutomation 2006 - SLAS

Where Laboratory Technologies Emerge and Merge
3:30 pm Tuesday, January 24, 2006 Track 2: Micro- and Nanotechnologies Room: Pasadena
Wyndham Palm Springs Hotel
Paul Bohn
University of Illinois
Urbana, Illinois
bohn@scs.uiuc.edu
Nanofluidics and Mass-Limited Chemical Analysis: Nanocapillary Array Membranes as
Switchable Fluidic Elements for Multidimensional Analyses
Motivated by problems posed by biothreat agents, a grand challenge problem for contemporary chemical analysis is the handling and
characterization of mass-limited samples. Our approach is to integrate nanometer-scale analytical unit operations into three-dimensional
architectures to create integrated fluidic circuits, i.e. structures which handle fluids with the same digital control protocols used by
integrated electronic circuits. We are exploring externally controllable interconnects, employing nanocapillary array membranes containing
1-104 nanometer diameter-channels, to produce hybrid three-dimensional fluidic architectures, in which controllable nanofluidic transfer is
achieved by controlling applied bias, polarity and density of the immobile nanopore surface charge, and the impedance of the nanopore
relative to the microfluidic channels. Such multi-level microfluidic structures are analogous to the massively three-dimensional architectures
characteristic of VLSI electronics and open the way for complex arrays of fluidic manipulations to be realized.
4:00 pm Tuesday, January 24, 2006 Track 2: Micro- and Nanotechnologies Room: Pasadena
Wyndham Palm Springs Hotel
Stephen C. Jacobson
Co-Author(s)
Indiana University
Zexi Zhuang
Bloomington, Indiana
jacobson@indiana.edu
Margaret A. Lerch
Multidimensional Separations of Peptides on Microfluidic Devices
For many of the electrokinetically driven separation techniques demonstrated on microfluidic devices, the separative performance
measured per unit length is similar to or exceeds that of conventional capillary separations. These high performance separations can also
be applied to separations in multiple dimensions. In part, high performance is maintained by precise sample handling between dimensions.
This can be achieved by rapid switching of the fluid streams and designing the channel architecture to effectively transition the sample
from the first to the second dimension. A serial-to-parallel format is being developed where the first dimension separation is conducted in
a single channel and the second dimension separation in an array of parallel channels. Also, a planar format is being considered where the
first and second dimension separations are both performed in a rectangular channel with a high aspect ratio. Device design, operation, and
performance will be discussed.
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