LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS
LabAutomation2006 10:30 am Wednesday, January 25, 2006 Track 1: Detection & Separation Room: Catalina Wyndham Palm Springs Hotel Joni Stevens Co-Author(s) Gilson, Inc. Greg Robinson Middleton, Wisconsin Luke Roenneburg jstevens@gilson.com Tim Hegeman Alan Hamstra Automated 2D HPLC Using Trap Columns for the Fractionation, Isolation and Screening of Natural Products One aspect of 2D chromatography that is being explored is to use small Reverse-Phase columns to trap the eluent from the Ion-Exchange column at defined timed intervals and then elute the components from the “trapping” Reverse-Phase columns onto a analytical RP column with fraction collection for further analysis techniques like mass spectroscopy and bioactivity assays. All types of 2D chromatography have an attractive characteristic in that they enhance the separation of a mixture that is just not available by 1D chromatography. However manual 2D chromatography can be difficult to repeat and operate, hence automation would alleviate the variables and tremendously increase repeatability and throughput. A resurgence in natural product evaluation for biological activity is evident based on articles noted in scientific journals and public media. One such natural product is Mugwort Artemisia vulgaris, or black sage, whose components have been associated with digestive stimulant, a diuretic, and nerve tonic. The main chemical components of Mugwort extracts are alpha, beta-thujones, 1,8-cineole, camphene and camphone which have associative affects of neurotoxicity and abortifacient. 10:30 am Monday, January 23, 2006 Track 2: Micro- and Nanotechnologies Room: Pasadena Wyndham Palm Springs Hotel Robert Dunn University of Kansas Lawrence, Kansas rdunn@ku.edu Optical Imaging Beyond the Classical Diffraction Limit Near-field scanning optical microscopy (NSOM) is a scanning probe technique that enables optical measurements to be conducted with nanometric spatial resolution. This technique offers single molecule detection limits, high spatial resolution, and simultaneous force and optical mapping of sample properties. As such, it has found applications in many areas including the study of thin films, polymers, and solid-state materials. Perhaps its greatest potential, however, lies in the biological sciences, where fluorescence techniques are well developed for tagging specific proteins or structures or following dynamic processes such as calcium signaling. Our laboratory has been actively developing NSOM methods that are amenable with soft and fragile samples such as living cells. The development of these techniques and their biological applications will be discussed. 60
Where Laboratory Technologies Emerge and Merge 11:00 am Monday, January 23, 2006 Track 2: Micro- and Nanotechnologies Room: Pasadena Wyndham Palm Springs Hotel Michael Natan Nanoplex Technologies, Inc. Mountain View, California mnatan@nanoplextech.com High Performance Optical Tags Based on Encapsulated SERS-Active Nanoparticles In life sciences, there is an urgent need for optical detection tags that (i) can be interrogated using near-IR wavelengths (where biological samples do not absorb or emit light), and (ii) can allow multiple species to be tracked simultaneously. Nanoplex’s patented SERS nanotags, comprising glass-encapsulated gold nanoparticles loaded with a series of reporter molecules, solve both these problems; in addition, they are designed to be straightforward to manufacture and extraordinarily stable. This presentation will describe their optical properties, both in bulk and at the single particle level, and highlight a series of applications, from multiplexed protein assays to in vivo imaging. 11:30 am Monday, January 23, 2006 Track 2: Micro- and Nanotechnologies Room: Pasadena Wyndham Palm Springs Hotel Robin Liu Co-Author(s) CombiMatrix Corp. Tai Nugyen Mukilteo, Washington Kevin Schwarzkopf rliu@combimatrix.com H. Sho Fuji Kia Peyvan David Danley Andy McShea F I N A L I S T Fully Integrated Microfluidic Devices for Automated DNA Microarray Analysis DNA microarray assays involve multi-stage sample processing and fluidic handling, which are generally labor-intensive and time-consuming. Using microfluidic technology to integrate and automate all these steps in a single device is highly desirable in many practical applications (e.g., point-of-care genetic analysis, disease diagnosis, and in-field bio-threat detection). We have developed self-contained and fully integrated microfluidic devices for DNA analysis. These disposable devices consist of microfluidic pumps, mixers, valves, channels/chambers, and Combimatrix microarray silicon chip. Microarray hybridization and subsequent fluidic handling and reactions (including a number of washing and labeling steps) and detection were performed in these devices. Transcriptional analysis of K562 cells with a series of spiked-in controls was performed to characterize this new platform with regard to sensitivity, specificity, and dynamic range. The device detected sample RNAs with a concentration as low as 0.375 pM. Detection was quantitative over three orders of magnitude. The devices are completely self-contained: no external pressure sources, fluid storage, mechanical pumps, mixers, or valves were necessary for fluid manipulation, thus eliminating possible sample contamination and simplifying device operation. All microfluidic components use very simple and inexpensive approaches in order to reduce chip complexity. In addition to fluorescence-based detection, an enzyme-based electrochemical detection method that has many advantages including high sensitivity (~fM) and simple apparatus was developed and integrated. The microfluidic devices with capabilities of on-chip sample processing and detection provide a cost-effective solution to eliminate labor-intensive and time-consuming fluidic handling steps that can be a significant source of variability in genomic analysis. 61
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<strong>LabAutomation</strong><strong>2006</strong><br />
10:30 am Wednesday, January 25, <strong>2006</strong> Track 1: Detection & Separation Room: Catalina<br />
Wyndham Palm Springs Hotel<br />
Joni Stevens<br />
Co-Author(s)<br />
Gilson, Inc.<br />
Greg Robinson<br />
Middleton, Wisconsin<br />
Luke Roenneburg<br />
jstevens@gilson.com<br />
Tim Hegeman<br />
Alan Hamstra<br />
Automated 2D HPLC Using Trap Columns for the Fractionation, Isolation and<br />
Screening of Natural Products<br />
One aspect of 2D chromatography that is being explored is to use small Reverse-Phase columns to trap the eluent from the Ion-Exchange<br />
column at defined timed intervals and then elute the components from the “trapping” Reverse-Phase columns onto a analytical RP column<br />
with fraction collection for further analysis techniques like mass spectroscopy and bioactivity assays. All types of 2D chromatography<br />
have an attractive characteristic in that they enhance the separation of a mixture that is just not available by 1D chromatography. However<br />
manual 2D chromatography can be difficult to repeat and operate, hence automation would alleviate the variables and tremendously<br />
increase repeatability and throughput. A resurgence in natural product evaluation for biological activity is evident based on articles noted in<br />
scientific journals and public media. One such natural product is Mugwort Artemisia vulgaris, or black sage, whose components have been<br />
associated with digestive stimulant, a diuretic, and nerve tonic. The main chemical components of Mugwort extracts are alpha,<br />
beta-thujones, 1,8-cineole, camphene and camphone which have associative affects of neurotoxicity and abortifacient.<br />
10:30 am Monday, January 23, <strong>2006</strong> Track 2: Micro- and Nanotechnologies Room: Pasadena<br />
Wyndham Palm Springs Hotel<br />
Robert Dunn<br />
University of Kansas<br />
Lawrence, Kansas<br />
rdunn@ku.edu<br />
Optical Imaging Beyond the Classical Diffraction Limit<br />
Near-field scanning optical microscopy (NSOM) is a scanning probe technique that enables optical measurements to be conducted<br />
with nanometric spatial resolution. This technique offers single molecule detection limits, high spatial resolution, and simultaneous force<br />
and optical mapping of sample properties. As such, it has found applications in many areas including the study of thin films, polymers,<br />
and solid-state materials. Perhaps its greatest potential, however, lies in the biological sciences, where fluorescence techniques are<br />
well developed for tagging specific proteins or structures or following dynamic processes such as calcium signaling. Our laboratory has<br />
been actively developing NSOM methods that are amenable with soft and fragile samples such as living cells. The development of these<br />
techniques and their biological applications will be discussed.<br />
60