LabAutomation 2006 - SLAS

Where Laboratory Technologies Emerge and Merge
11:00 am Monday, January 23, 2006 Track 1: Detection & Separation Room: Catalina
Wyndham Palm Springs Hotel
Anthony Tsarbopoulos
Co-Author(s)
University of Patras
Fotini Bazoti
GAIA Research Center
Kifissia, Greece
University of Patras
atsarbop@gnhm.gr
Jonas Bergquist
Karin Markides
Uppsala University
Evaluation of Natural Products Towards the Prevention and Treatment of
Alzheimer’s Disease
The continuing demographic shift of population towards an older society has led to a growing prevalence of chronic age-related diseases in
all industrialized countries. Development of degenerative diseases, such as Alzheimer’s Disease (AD), associated with neurodegeneration,
massive brain cell loss, loss of cognitive ability and premature death, has a major impact on health along with economical ramifications
in Western world. Even though the cause of AD remains ambiguous, one of the prevailing hypotheses has centered on the amyloid beta
protein (Aâ)-containing senile plaques, with oxidative stress being the main mechanism proposed to justify Áâ’s aggregation.
In light of the suggested link between oxidative stress and AD, it is proposed that endogenous antioxidants or dietary derived compounds
may offer an ideal therapeutic regime for protection against the risk of this disease. In this presentation, the formation of noncovalent
complexes of Aâ with endogenous antioxidants, such as melatonin, and certain bioactive phytochemicals, derived from plants endemic
in Mediterranean flora, has been demonstrated by electrospray ionization mass spectrometric (ESI MS) analysis. Several experimental
parameters which affect the stability and specificity of the noncovalent complexes have been examined, while the binding site of the
antioxidant on Aâ has been identified by proteolytic mapping combined with FTICR-ESI MS analysis. These data along with NMR data on
the Aâ residues which are involved in the noncovalent interaction may shed some light into the mechanisms of AD pathology and provide
insights into novel agents that can be employed towards prevention or even treatment of AD.
11:30 am Monday, January 23, 2006 Track 1: Detection & Separation Room: Catalina
Wyndham Palm Springs Hotel
Gary Kruppa
Co-Author(s)
Bruker Daltonics Inc.
Manfred Spraul
Fremont, California
Peter Neidig
gary.kruppa@bdal.com
Hartmut Schaefer
Bruker Biospin
Gabriela Zurek
Carsten Baessmann
Bruker Daltonik
Investigation of Metabolite Profiles in Human Urine by ESI-oaTOF and
Quadrupole Ion Trap MS
The quantitative measurement of the time-related multi-parametric metabolic response of living systems to pathophysiological stimuli
or genetic modification is of great current interest for possible applications in biomarker discovery, clinical diagnostics and other areas.
Measuring metabolites in urine is of special interest since metabolic endpoints can be monitored and urine samples can be obtained
non-invasively from animals and humans. We describe here an automated and high-throughput method for extracting this important
biochemical information using mass spectrometry. The method uses accurate mass data from a high resolution ESI-TOF, which provides a
tool to directly generate molecular formulas of metabolites. This data is analyzed using multivariate statistical tools e.g. principal component
analysis. Retention time information combined with accurate mass data are used to identify important biochemicals, which are shown
to be statistically significant in describing the changes to the living system. MSn data adds complementary structural information when
identification cannot be made using the molecular formula and retention time. In order to obtain consistent and meaningful statistical data
from the typically noisy LC-MS chromatograms, an appropriate method for extracting peaks from the chromatograms is required. We have
been evaluating various chromatographic peak finding techniques for this purpose.
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