LabAutomation 2006 - SLAS

LabAutomation2006
1:30 pm Wednesday, January 25, 2006 Plenary Session Room: Primrose Ballroom
Palm Springs Convention Center
E.L. Kersten
Despair, Inc.
Demotivation: The State of the Art
After intense, thought-provoking days of discussion, end your LabAutomation2006 experience with a less intense, yet still thoughtprovoking,
discussion of the art of demotivation. It’ll make you think. But, more importantly, it’ll make you laugh.
Explore this mirthful topic with E. L. Kersten, who started his career as a university professor, but left academia to join an internet startup.
We all know how that field ended up. Needless to say, his experience there was tumultuous and transformational, ultimately inspiring the
birth of Despair, Inc.
Kersten promises a review of how visionary companies are using demotivational techniques to transform their workforces. Do they truly
work? You be the judge …
10:30 am Monday, January 23, 2006 Track 1: Detection & Separation Room: Catalina
Wyndham Palm Springs Hotel
Christopher L. Hendrickson
Co-Author(s)
Florida State University
Greg Blakney
Tallahassee, Florida
Mark Emmett
hendrick@magnet.fsu.edu
Sasa Kazazic
John Quinn
Ryan P. Rodgers
Tanner M. Schaub
Alan G. Marshall
National High Magnetic Field Laboratory
Automated Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Ultrahigh
Resolution and Part-per-Billion Mass Accuracy
We describe automated analysis of multiple samples by high field (9.4 and 14.5 tesla) Fourier transform ion cyclotron resonance (FT-ICR)
mass spectrometry. Mass resolving power is routinely greater than 100,000 at 1 Hz scan rate, and can exceed one million as necessary.
Accurate mass can be achieved by use of an internal calibrant (typically ~100 parts-per-billion rms error for petroleum samples at 9.4 T) or by
combination of external calibration and automatic gain control (AGC) of the number of ions injected into the ICR trap (typically ~500 ppb for
broadband and >50 ppb for single ion monitoring at 14.5 T). Several MS/MS fragmentation techniques are available and complementary,
including collisionally-activated (CAD in the linear trap), infrared multiphoton (IRMPD in the ICR cell), and electron capture dissociation
(ECD in the ICR cell). Rapid dissociation and sensitive mass selection facilitate MS/MS at 1 Hz while high field maintains ultrahigh resolving
power. Representative applications include petrochemical analysis in support of deepwater oil production and proteome profiling (with
an electrospray robot), and hydrogen-deuterium exchange LC MS for elucidation of protein binding sites (with programmable sample
handling). Work supported by NSF CHE-99-09502, Thermo Electron Corporation, Florida State University, and the National High Magnetic
Field Laboratory in Tallahassee, FL.
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