LabAutomation 2006 - SLAS
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LabAutomation 2006 - SLAS

LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS

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TP89 Wanli Xing Tsinghua University School of Medicine Beijing, China wlxing@tsinghua.edu.cn LabAutomation2006 Co-Author(s) Dong Liang, Tsinghua University Wanli Xing, Tsinghua University School of Medicine Qiang Peng, Tsinghua University Zhongyao Yu, National Engineering Research Center for Beijing Biochip Technology Jing Cheng, Tsinghua University School of Medicine The Design and Development of a Gas Chromatography Column Chip With High Efficiency The design, fabrication, and performance of a gas chromatography (GC) column chip with high efficiency are described. Wet chemical etching formed a 3.46 m ¡Á 100 ¦Ìm ¡Á 48 ¦Ìm channel on a silicon wafer. A Pyrex glass wafer was anodically bonded to the silicon, forming an intact column. Fused silica capillary connecting tubes were sealed into the laser-drilled inlet and outlet of the chip. A dynamic coating method was used to deposit a film of nonpolar dimethyl polysiloxane stationary phase, SE-30. The chip was evaluated using a commercial GC instrument. The height equivalent to a theoretical plate (HETP) under the pressure of 55 psi was 0.30 mm for n-octane and 0.29 mm for n-nonane, respectively. Both regression and comparison calculation led to the same conclusion that a rather low Hmin have been achieved. In conclusion, we have made a GC column chip with greatly improved efficiency. TP90 Keith Albert Artel Marketing R&D Westbrook, Maine kalbert@artel-usa.com Co-Author(s) John Thomas Bradshaw Tanya R. Knaide Alexis L. Rogers Verifying Volume Dispensing Device Performance for Complex and/or Non-Aqueous Reagents: A New Approach Multichannel volume dispensing devices, such as automated liquid handling (ALH) systems, are widely used in drug discovery assays and other high-throughput screening processes. The performance of these systems is heavily based on the ability to deliver proper volumes of specific reagents. For instance, because concentrations of species within an assay are volume-dependent, assay integrity and the subsequent interpretation of assay results are directly tied to ALH performance. While ALH instruments are capable of handling a wide array of reagent types, it is commonly known that performance parameters can vary significantly when the reagents are complex in nature. When ALH systems are employed to aspirate/dispense aqueous-based reagents, there are many accepted methodologies (including photometric and gravimetric) used to calibrate/verify the system’s ability to properly perform within a user’s tolerance window. In other situations, however, ALH systems are employed to dispense complex and/or non-aqueous reagents (dimethyl sulfoxide, serum, aqueous-based mixtures with detergents, etc.) for which there are fewer accepted methodologies to verify system performance. ALH software packages incorporating computational algorithms may provide users with the ability to adjust aspirate/dispense parameters to help compensate for liquid-dependent performance differences, but these parameters could lead to a false-sense of performance when a custom, or complex, reagent is employed. We present our recent research on developing a novel photometric method for ALH calibration and volume verification when dispensing complex and/or non-aqueous reagents. Accurate and reliable adjustment of ALH performance using this method could have far-reaching adoption in all scientific communities for any volume dispensing device. 196

TP91 Casey Williams Agencourt Bioscience Corporation Beverly, Massachusetts cwilliams@agencourt.com Where Laboratory Technologies Emerge and Merge Co-Author(s) Olaf Stelling, Dustin Giberson, Kimberly Sparks, Lakeisha Tillery Martina Werner, Erik Gustafson, Agencourt Bioscience Corp., A Beckman Coulter Company Kelly Marshall, Laura Pajak Beckman Coulter, Inc. An Automated Method for the Isolation of Genomic DNA from Whole Blood using Beckman Coulter’s Biomek ® Laboratory Automation Workstations and Agencourt ® GenfindTM DNA Isolation Kit Isolation and purification of nucleic acids is a crucial step in basic research, molecular diagnostics and pharmacogenomics applications. Whole blood is commonly used for the extraction of genomic DNA in clinical research settings, but presents many challenges in sample preparation. Blood is rich in proteins, lipids and other cellular materials that need to be effectively removed to isolate high quality genomic DNA. Traditional methods have relied on separation of white blood cells via density gradient centrifugation and extraction with harsh organic chemicals. Column purification techniques exist, but are not easily automated. We present a scalable, 96-well, automatable method for effectively isolating large quantities of genomic DNA from fresh or frozen whole blood using the magnetic-bead based, Solid Phase Reversible Immobilization (SPRI ® ) technology in the Genfind DNA Isolation Kit.. Furthermore, sufficient genomic DNA can be isolated from serum samples for amplification-based analyses. This SPRI-based process is easily automated to produce high yield, high quality genomic DNA from whole blood. We present automation methods that can process up to 200 µL of whole blood or serum per well when introduced onto the system in a 96-well format. An entire plate of samples can be processed using a multi-channel Biomek FX Laboratory Automation Workstation or 1 to 96 samples in sets of 8 (per column) using a Span-8 Biomek NX Laboratory Automation Workstation. TP92 Justin Murray Merck & Co. Inc. North Wales, Pennsylvania justin_murray@merck.com Co-Author(s) Jason Cassaday Phil Moravec Merck & Co. Inc. Carissa Ohart Kelly Scientific Resources Tarak Shah Merck & Co. Inc 1536-Well Non-Contact Dispense of YOx Imaging SPA Beads Yttrium Oxide (YOx) imaging SPA beads are more desirable to use than plastic beads (PS - Poly Styrene). However, YOx beads are also 4 times as dense making them very difficult to keep in suspension and dispense without well to well concentration variance. We have created a way to keep the beads in suspension for extended periods of time. We have also developed a method for quickly calculating bead concentration without the use of radioactive materials. Using these practices we have optimized two dispensers for YOx SPA bead non-contact dispense into 1536 well format – the Kalypsys SPA Bead Dispenser and the Cartesian Dispenser. We will compare these QC results from each dispenser along with a comparison using real assay data. 197

TP91<br />

Casey Williams<br />

Agencourt Bioscience Corporation<br />

Beverly, Massachusetts<br />

cwilliams@agencourt.com<br />

Where Laboratory Technologies Emerge and Merge<br />

Co-Author(s)<br />

Olaf Stelling, Dustin Giberson, Kimberly Sparks, Lakeisha Tillery<br />

Martina Werner, Erik Gustafson,<br />

Agencourt Bioscience Corp., A Beckman Coulter Company<br />

Kelly Marshall, Laura Pajak<br />

Beckman Coulter, Inc.<br />

An Automated Method for the Isolation of Genomic DNA from Whole Blood using<br />

Beckman Coulter’s Biomek ® Laboratory Automation Workstations and Agencourt ®<br />

GenfindTM DNA Isolation Kit<br />

Isolation and purification of nucleic acids is a crucial step in basic research, molecular diagnostics and pharmacogenomics applications.<br />

Whole blood is commonly used for the extraction of genomic DNA in clinical research settings, but presents many challenges in sample<br />

preparation. Blood is rich in proteins, lipids and other cellular materials that need to be effectively removed to isolate high quality genomic<br />

DNA. Traditional methods have relied on separation of white blood cells via density gradient centrifugation and extraction with harsh<br />

organic chemicals. Column purification techniques exist, but are not easily automated. We present a scalable, 96-well, automatable<br />

method for effectively isolating large quantities of genomic DNA from fresh or frozen whole blood using the magnetic-bead based, Solid<br />

Phase Reversible Immobilization (SPRI ® ) technology in the Genfind DNA Isolation Kit.. Furthermore, sufficient genomic DNA can be isolated<br />

from serum samples for amplification-based analyses. This SPRI-based process is easily automated to produce high yield, high quality<br />

genomic DNA from whole blood. We present automation methods that can process up to 200 µL of whole blood or serum per well when<br />

introduced onto the system in a 96-well format. An entire plate of samples can be processed using a multi-channel Biomek FX Laboratory<br />

Automation Workstation or 1 to 96 samples in sets of 8 (per column) using a Span-8 Biomek NX Laboratory Automation Workstation.<br />

TP92<br />

Justin Murray<br />

Merck & Co. Inc.<br />

North Wales, Pennsylvania<br />

justin_murray@merck.com<br />

Co-Author(s)<br />

Jason Cassaday<br />

Phil Moravec<br />

Merck & Co. Inc.<br />

Carissa Ohart<br />

Kelly Scientific Resources<br />

Tarak Shah<br />

Merck & Co. Inc<br />

1536-Well Non-Contact Dispense of YOx Imaging SPA Beads<br />

Yttrium Oxide (YOx) imaging SPA beads are more desirable to use than plastic beads (PS - Poly Styrene). However, YOx beads are also<br />

4 times as dense making them very difficult to keep in suspension and dispense without well to well concentration variance. We have<br />

created a way to keep the beads in suspension for extended periods of time. We have also developed a method for quickly calculating<br />

bead concentration without the use of radioactive materials. Using these practices we have optimized two dispensers for YOx SPA bead<br />

non-contact dispense into 1536 well format – the Kalypsys SPA Bead Dispenser and the Cartesian Dispenser. We will compare these QC<br />

results from each dispenser along with a comparison using real assay data.<br />

197

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