LabAutomation 2006 - SLAS

TP81
Wayne Bowen
TTP LabTech
Melbourn, Hertfordshire, United Kingdom
wayne.bowen@ttplabtech.com
LabAutomation2006
Co-Author(s)
Ben Schenker
TTP LabTech
Ian Yates
Velocity11
A Total Solution to Provide High Content Primary and Secondary Screening of a
Compound Library
This is because primary screens need to be fast, low cost and generate limited information. In contrast, secondary screens must provide
robust, detailed information, requiring the application of more sophisticated liquid handling and detection capabilities.
Advances in automation, integration, scheduling and high-content analysis technology have enabled the design of a single system to
perform both the primary and secondary screens of a compound library. The primary screen process involves the initial selection of
compounds from a library, which are sampled in assay-ready volumes to produce 384 well plates. Assay constituents and cells are added
prior to incubation with test compound, followed by high content read-out using an Acumen Explorer microplate cytometer, giving a daily
throughout of over 100,000 compounds.
The secondary screen process involves cherry-picking the hits from the primary screen and generating dose response curves for each one,
again in assay-ready volumes. The plates are prepared as before and analysed using an Acumen Explorer.
G-protein coupled receptors (GPCR) represent the largest and most frequently screened class of receptors. TTP LabTech recently
published a novel approach combining the Acumen Explorer with Invitrogen’s GeneBLAzer technology to measure GPCR activation in
cell-based assays. Here, we show how this approach could be fully automated by the system described to complete the primary and
secondary screens on 400,000 compounds in less than a week.
TP82
Chris Bridge
DNA Research Innovations Ltd
Kent Science Park, United Kingdom
chris.bridge@invitrogen.com
Co-Author(s):
A. Potts
T. Stevenson,
M.Baker
Fully Automated Extraction of gDNA From up to 10mL Whole Blood
Recent advances in the fields of molecular screening, diagnostics, and clinical applications have led to increased demand for high quality
dna purification techniques from whole blood (clinical) samples. Here we present a fully-automated, scalable gDNA extraction method,
purifying high quality gDNA from up to 10mL whole blood. The centrifuge-free, single-tube protocol has been fully automated, from blood
collection tube to purified dna on a Tecan Freedom Evo automated liquid handler.
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