LabAutomation 2006 - SLAS

TP75
Julian Willmott
White Carbon
Royston, Herts, United Kingdom
julian.willmott@white-carbon.com
Where Laboratory Technologies Emerge and Merge
Pathways for Biological Reagent Quality and Workflow Tracking (CIMS)
Biological reagents are the single most important factor in the success of assays. Use of poor or unsuitable biological materials can lead
to huge wastage of time and resources. CIMS is a novel system that provides a unique combination of active tracking in the laboratory
and desktop analysis software. It provides laboratories with easy-to-use data portals for gathering cell quality data such as cell viability, cell
density and passage number. It associates these reagent quality data with plates and tracks their progress around the lab. Once assay
screening has been performed, the screening results for plates are merged with the reagent quality data. The CIMS viewer application can
then be used to quickly and easily identify whether poor or unexpected screening results were caused by biological factors.
The poster will show the CIMS laboratory and desktop applications, how they integrate with instrumentation (such as SelecT cell culture)
and how they integrate with existing IT systems (such as Spotfire).
TP76
Tracy Worzella
Promega Corporation
Madison, Wisconsin
tracy.worzella@promega.com
Co-Author(s)
Brad Larson
Aoife Gallagher
Phillip Hassell
Deerac Fluidics
Kinase Assay Optimization and Profiling in uHTS Format
Kinases play a crucial role in regulating complex cellular processes including activation, growth, and differentiation. Considering this, it is
not surprising that abnormal kinase activity or function can lead to a variety of human diseases including cancer, arthritis, and diabetes.
Pharmaceutical companies are focusing efforts more than ever to find the next kinase inhibitor in their compound librairies. Companies look
to assay miniaturization to do this cost effectively, yet obtain value-added information from assay development and screening activities.
We demonstrate the use of Promega’s Kinase-Glo ® Plus Luminescent Kinase Assay for miniaturized kinase assays using Deerac Fluidics’
Equator HTS liquid handling system. The Kinase-Glo ® Plus Assay is a homogeneous method of measuring kinase activity by quantifying
ATP in solution following a kinase reaction. The Equator HTS is a non-contact liquid dispenser capable of delivering volumes from 20ul
down to 50nl.
Because assay development and screening are key uses for miniaturized assays, we show the Equator HTS can be used to optimize
Kinase-Glo ® reactions, and screen for kinase activity. We use the Equator HTS to titrate kinases, substrates and ATP to determine optimal
concentrations for subsequent screening activities in 1536-well format. A panel of kinases and compounds are counter-screened for kinase
selectivity profiling. 24-point serial titration is performed to determine potency. Data show that miniaturization of the Kinase-Glo ® Plus Assay
does not compromise assay quality. The combination of Kinase-Glo ® Plus and the Equator HTS provides an integrated solution for kinase
drug discovery.
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