LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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TP69<br />
Paige Vinson<br />
Thermo Electron Corporation<br />
Brentwood, Tennesee<br />
paige.vinson@Thermo.com<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Description of a “Data-centric” Approach to a Fully Integrated Protein<br />
Crystallization System<br />
Crystallization is a process within crystallography that consists of many trials before observing the desired outcome. Each experiment is<br />
comprised of several separate, yet linked, steps that are associated with each other through the data and results that each generates.<br />
The retrieval of these data is necessary for the thorough evaluation of experimental results and for refinement of experimental conditions.<br />
Presented here is a description of a “data-centric” approach through which all the data that are entered and generated are available to<br />
aid the user in observing trends in the data, determining future experimental conditions, as well as configuring and controlling hardware<br />
components involved in the process. Part of this approach includes a modular and highly integrated set of software that provides an<br />
interface enabling the user to enter and retrieve data from the database. The software also guides the crystallographer from the planning<br />
stage through to the refinement of the experimental process including data mining using an intuitive tool for this purpose. These and other<br />
features of the software will be presented in a format describing typical scenarios and methods of use including remote resource sharing.<br />
TP70<br />
Leslie Walling<br />
Amgen Inc.<br />
Thousand Oaks, California<br />
lwalling@amgen.com<br />
Effective Mixing in 384-Well Micro Titer Plates<br />
Co-Author(s)<br />
Craig Schulz<br />
Tim Romig<br />
Mike Johnson<br />
Nelson Carramanzana<br />
Amgen<br />
Mixing in SBS standard 384-well plates has proven quite different from 96-well and other larger well formats. The aspect ratio of a typical<br />
well, the balance of surface tension and mass of the fluids and the scale of diffusion all add to the increased difficulty in mixing fluids in<br />
higher density plates. Here we will introduce methods to quantify the effectiveness of mixing in 384-well plates. The methods are used<br />
to benchmark the performance of several commercial shakers. Additionally some high-performance techniques are introduced where<br />
complete well mixing is attainable in seconds.<br />
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