LabAutomation 2006 - SLAS

TP67
Kathleen Tivel
Pfizer Global R&D
San Diego, California
kathleen.tivel@pfizer.com
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Yunwen Chiu
Loanne Chung
Brad De Bruler
Christine Aurigemma
Jeff Elleraas
William Farrell
Recent Developments in High-Throughput Analysis and Purification by LC/MS and
SFC/MS at Pfizer La Jolla
Combinatorial chemistry technologies, including parallel synthesis and high-throughput analysis techniques, allow rapid synthesis and
screening of potential drugs. Pfizer’s High-Throughput Discovery facility in La Jolla produces purified small-molecule targeted libraries via a
highly integrated system of instrumentation, custom software and databases. While this is our primary focus, we are often called upon to
provide other analytical and preparative services to allow projects to move forward with critical compounds of interest that do not fit into the
process model.
Our technology includes high-throughput LC/MS, SFC/MS, UV- and MS-based preparative LC and UV-based preparative SFC. These
complementary techniques enabled purification of tens of thousands of compounds at the rate of up to 2,000 per week. Purity, assessed
by ELSD, UV, APCI-MS, and NMR is over 85%, with a success rate generally over 60%.
A single, universal analytical method is desirable, but due to the diversity of compounds produced, multiple methods are required to produce
quality compounds for screening. We present recent experiments exploring technologies to ensure realistic assessment of libraries while
maximizing success of preparative HPLC and SFC and increasing throughput. These include monolithic columns for analysis and purification,
nitrogen chemiluminescence detection (NCD), novel columns for SFC analysis and purification and rapid unattended method screening.
TP68
Mark Truesdale
Genetix
New Milton, United Kingdom
mark.truesdale@genetix.com
Co-Author(s)
Irene Bramke
Chris Mann
Julian F Burke
The Optimisation of Semi-Solid Media for High-Throughput Screening and Picking of
Mammalian Cells
The ability to increase the through-put of mammalian cell screening, and shorten the discovery process, is critical to all Biopharmaceutical
companies. The automated screening and selective picking of clonal cell colonies direct from media offers a solution to a bottle neck in
this process. The use of semi-solid media confers positional integrity on the growing cell colonies and therefore can facilitate automated
screening and picking of the colonies using criteria based upon protein secretion or morphology. We will present data on semi-solid media
that allows the transition from liquid to semi-solid cell culture for a range of different cell types.
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