LabAutomation 2006 - SLAS

TP63
Andreas Stelzer
Thermo Electron
Burlington, Ontario, Canada
andreas.stelzer@thermo.com
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Marta Kozak
Robert DeWitte
Robert Dunn-Dufault
Hansjoerg Haas
High-Throughput Intrinsic Clearance Studies with Thermo’s LeadStream
ADME/Tox Solution
ADME/Tox screening continues to play an integral role in the drug development process with more assays putting higher throughput
demands onto screening. The rate of metabolism, measured as intrinsic clearance is one such assay. With the multiple samplings required
and subsequent increased plate handling the current capacities of manual and semi-automated procedures is being strained. LeadStream
enables this assay to be fully automated with out trading off quality for quantity. Utilizing the complete LeadStreamTM platform, WorkCell,
LC/MS, and Reformattor, we have performed high throughput in vitro intrinsic clearance studies with human microsomal preparations. In
this poster the LeadStream performance for this assay will be reported and compared to typical literature values. With the minimal operator
assistance a LeadStream system can easily fulfill the demands of today and tomorrow.
TP64
Joni Stevens
Gilson, Inc.
Middleton, Wisconsin
jstevens@gilson.com
Co-Author(s)
Mark muncey
Greg Robinson
Norbert Wodke
Automated Fast-Bead Synthesis of Small Peptides
In proteomic research the synthesis of peptides in small quantities is required for “fast and quick assays”. These assays provide possible
hits that can be synthesized at greater concentrations later. The synthesis of these peptides is usually in the 1 mg range and requires
hundreds of peptides to be synthesized at a time. The application presented offers a novel yet simple automated system to accomplish this
task. An automated liquid handler is equipped with 96-well filter plates attached to a vacuum system/rack. The resin has been manually
added to the individual wells of the filter plate. Employing the capabilities of the liquid handler reagents, solvents, and amino acids are
added to the resin, based on the order and volume defined by the researcher. This information is quickly accessed through a text file into
the program. A series of activated valves allows positive pressure to be applied to the plates, in order to maintain the interaction with the
resin in the wells and the added components. Valves access the vacuum capabilities of the rack to withdrawal the solutions through the
resins. The individual steps, capabilities and synthesis of a series of peptides via this automated system will be presented.
183