LabAutomation 2006 - SLAS

TP61
Robert Stanaker
Perkin Elmer
Downers Grove, Illinois
rob.stanaker@perkinelmer.com
LabAutomation2006
Co-Author(s)
Len Dugad
James Clark
Robert Stanaker
High Throughput Nanoliter Dispensing Using NanoHead Technology
The NanoHead is an optional dispense head for the Evolution P3 automated precision liquid dispensing platform for use in HTS and
cell based assays. It can dispense compounds, nucleic acids, proteins and antibodies in the 50 nL to 1 µL volume range. The Evolution
P3 with Modular Dispense Technology (MDT) allows automated exchange of dispense heads enabling dynamic range of dispensing from
50 nL to 235 µL without manual intervention. Dispense accuracy, precision, and sample carryover results are presented for typical highthroughput
screening, genomics and proteomics applications. The NanoHead can be used for dispensing drug compounds dissolved in
DMSO to minimize DMSO content in the final assay reaction mixture.
TP62
Olaf Stelling
Agencourt Bioscience Corp.
Beverly, Massachusetts
ostelling@agencourt.com
Co-Author(s)
Dustin Giberson, Kimberly Sparks,
Agencourt Bioscience Corp., A Beckman Coulter Company
Lakeisha Tillery, Martina Werner, Erik Gustafson
Agencourt Bioscience Corp., A Beckman Coulter Company
Kelly Marshall, Laura Pajak
Beckman Coulter, Inc.
An Automated Method for the Isolation of Genomic DNA from Whole Blood using
Beckman Coulter’s Biomek ® Laboratory Automation Workstations and Agencourt’s ®
GenfindTM DNA Isolation Kit
Isolation and purification of nucleic acids is a crucial step in basic research, molecular diagnostics and pharmacogenomics applications.
Whole blood is commonly used for the extraction of genomic DNA in clinical research settings, but presents many challenges in sample
preparation. Blood is rich in proteins, lipids and other cellular materials that need to be effectively removed to isolate high quality genomic
DNA. Traditional methods have relied on separation of white blood cells via density gradient centrifugation and extraction with harsh organic
chemicals. Column purification techniques exist, but are not easily automated. We present a scalable, 96-well, automatable method
for effectively isolating large quantities of genomic DNA from fresh or frozen whole blood using the magnetic-bead based, Solid Phase
Reversible Immobilization (SPRI ® ) technology in the Genfind DNA Isolation Kit.. Furthermore, sufficient genomic DNA can be isolated from
serum samples for amplification-based analyses. This SPRI-based process is easily automated to produce high yield, high quality genomic
DNA from whole blood. We present automation methods that can process up to 200 µL of whole blood or serum per well when introduced
onto the system in a 96-well format. An entire plate of samples can be processed using a multi-channel Biomek FX Laboratory Automation
Workstation or 1 to 96 samples in sets of 8 (per column) using a Span-8 Biomek NX Laboratory Automation Workstation.
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